HPLC法测定不同来源不同采收期忍冬藤和金银花中绿原酸含量

Determination of Chlorogenic Acid in Honeysuckle Stem and Honeysuckle from Different Sources in Different Harvest Periods by HPLC

目的：采用高效液相色谱法测定不同来源不同采收期忍冬藤和金银花中绿原酸的含量。方法：色谱柱：Waters C18（250 mm×4.6 mm,5μm）;流动相：乙腈-0.4%磷酸溶液（10∶90）;检测波长：327 nm;流速：1.0 ml·min^-1;柱温：30℃。以外标法测定6个不同采收期、3种来源的忍冬藤和金银花中绿原酸的含量。结果：绿原酸进样量在0.065-1.300μg范围内与峰面积呈良好的线性关系（r=0.999 8）。9、10月采收的忍冬藤中绿原酸的含量最高,三种来源的忍冬藤中无毛淡红忍冬藤中绿原酸的含量最高。结论：忍冬藤中绿原酸的含量高低与采收时间有一定的关系,本研究为忍冬藤药材的采收加工提供了一定的理论依据。

Objective： To establish an HPLC method for the determination of chlorogenic acid in honeysuckle stem and honeysuckle from different sources in different harvest periods. Methods： A Waters C18column（ 250 mm × 4. 6 mm,5 μm） was used. The mobile phase consisted of acetonitrile-0. 4% H3PO4（ 10∶ 90） with the flow rate of 1. 0 ml·min^-1. The detection wavelength was 327 nm and the column temperature was 30℃. An external standard method was established for the determination of chlorogenic acid in honeysuckle stem and honeysuckle in six different harvest periods from three sources. Results： There was a good linear relationship within the range of 0. 065-1. 300 μg for chlorogenic acid（ r = 0. 999 8）. The content of chlorogenic acid in honeysuckle was the highest in September and October. The content of chlorogenic acid in Lonicera acuminata was the highest among the honeysuckle stem from three different sources. Conclusion： The content of chlorogenic acid in honeysuckle has a certain relationship with the harvest time,which can provide theoretical basis for the choice of harvest time for honeysuckle stem.