超高效液相色谱法研究影响二氯噁菌唑在土壤中降解因素

Study on Factors of Affecting Degradation of 2-Methyl sulfone-5-(2,4-dichlorophenyl)-1,2,3-oxadiazole in Soils by UPLC

建立了超高效液相色谱（UPLC）检测二氯噁菌唑在土壤中残留量的方法。在0.01～1.0 mg/kg添加水平范围内,二氯噁菌唑的平均添加回收率为94.80～99.65%,相对标准偏差（RSD）为4.1～8.1%,小于9%（n=5）;采用单因素控制变量法研究影响二氯噁菌唑在土壤中降解的主要因素：湿度、温度、土壤p H值、是否灭菌4个因素。结果表明,不同的湿度对二氯噁菌唑的降解影响不大,而不同的温度、p H值和灭菌对二氯噁菌唑的影响比较明显,土壤的p H值是影响二氯噁菌唑降解行为的主要因素。

A method for determining 2-methyl sulfone-5-（2,4-dichlorophenyl）-1,2,3-oxadiazole residue in soil was established based on ultra performance liquid chromatography（UPLC）, the residual level of this pesticide in soil samples was determined by UPLC. The results show that, when spiked level of 2-methyl sulfone-5-（2,4-dichlorophenyl）-1,2,3-oxadiazole is between 0.01～1.0 mg/kg, the recovery is 94.80%～99.65%, the relative standard deviation（RSD） is 4.1%～8.1%, less than 9%（n= 5）. The main four factors for 2-methyl sulfone-5-（2,4-dichlorophenyl）-1,2,3-oxadiazole degradation were investigated, including microbiological deterioration, soil humidity, temperature and p H value. The results show that soil p H is key factor.