马来丝虫微丝蚴定量注射感染中华按蚊和致倦库蚊的实验观察

OBSERVATIONS ON THE DEVELOPMENT OF QUANTITATIVELY INOCULATED BRUGIA MALAY I MICROFILARIAE IN ANOPHELES SINENSIS AND CVLEX QUINQUEFASCIATUS

本文报告采用改进的微量注射技术和较为精细的计数方法,以2—10条微丝蚴/蚊,定量注射感染中华按蚊和致倦库蚊,找出了这两种蚊虫的微丝蚴感染量与感染期幼丝虫阳性率、幼丝虫平均发育成熟率之间的定量关系;比较了在注射感染条件下两种蚊虫对马来丝虫易感性的差异。

With an improved technique, quantitative inoculation of Brugia malayl microfilariae (mff) into Anopheles sinensis and Culex quinquefasciatus was carried out in our laboratory in 1988. The development of B. malayi mff in these two species of mosquitoes was observed. In the system of B. malayi and An; sinensis, a well-fitted linear correlation appeared between the number of mff inoculated and the infective larva (L3) positive rate (r= 0.9910; P<0.01). The regression equation with the regression coefficient of 8.490 sugj gested a high susceptibility of this species of mosquito to B. malayi even under the condition of experimental inoculation. The quantitative relationship between the number of mff inoculated and the average filarial maturity rate was also exhibited. At the same dosages of 4 and 10 mff/mosquito, the L3 positive rates and the average filarial maturity rates in An. sinensis were much higher than those in Cx. quinquefasciatus (P<0.01), indicating significant difference occurred inside the bodies of these two species of mosquitoes.