活血通督汤对脊髓缺血再灌注损伤NF-κB、VCAM-1表达的作用

Effects of Huoxue Tongdu Decoction on inflammatory factor NF-κB and VCAM-1 during spinal cord ischemic-reperfusion injury

目的:观察活血通督汤对脊髓缺血再灌注损伤关键因子核因子-κB(NF-κB)、血管细胞黏附分子-1(VCAM-1)表达的影响,探讨活血通督汤对脊髓缺血再灌注损伤作用机制。方法:将新西兰家兔随机分为假手术组(8只)、模型组(32只)和活血通督汤组(32只)。3组术前7d均予连续灌胃,活血通督汤组灌服活血通督汤,每日2次,假手术组及模型组予灌服0.9%氯化钠溶液,每日2次。假手术组分离出L3-6动脉但不予夹闭,其余2组分离并夹闭L3-6动脉,制成脊髓缺血再灌注损伤模型。3组分别于术后0.5、1、4、8h取出家兔脊髓,采用免疫组化法和ELISA法检测脊髓组织中的NF-κB、VCAM-1的含量。结果:NF-κB在同一时点表达,假手术组最少,模型组最高;与假手术组相比,其余2组各时点NF-κB都明显升高(P<0.01);但活血通督汤组的表达水平显著低于模型组(P<0.05,P<0.01);VCAM-1在同一时点,假手术组表达最少,模型组表达最高;与假手术组相比,其余2组各时点VCAM-1的表达显著升高(P<0.01);血管表达率情况观察:假手术组始终无阳性血管表达,0.5h时3组皆无阳性血管表达,1、4、8h模型组阳性血管表达率明显高于活血通督汤组(P<0.05,P<0.01)。结论:活血通督汤能有效改抑制NF-κB、VCAM-1的表达,从而减轻炎性反应,以期达到减轻脊髓缺血再灌注损伤。

Objective： To observe the effects of Huoxue Tongdu Decoction on inflammatory factor NF-κB and VCAM-1 during spinal cord ischemic-reperfusion injury and explore its mechanism.Methods： New Zealand rabbits were randomly divided into three groups： sham-operation group（n=8）,normal saline group（n=32） and Huoxue Tongdu Decoction group（n=32）.For 7 days of pre-operation,Huoxue Tongdu Decoction group was gavaged with Huoxue Tongdu Decoction twice a day,while sham-operation group and normal saline group were both gavaged with normal saline twice a day.The sham-operation group was separated the L3-6 artery but not clipping,while other groups were separated the L3-6 artery to establish the ischemia reperfusion injury model of spinal cord.At 0.5,1,4,8 hours of post-operation,spinal cord of all rabbits were extracted.The immunohistochemistry method and ELISA were used to detect the contents of NF-κB and VCAM-1 in spinal cord.Results： The expression of NF-κB in sham-operation group was the lowest,normal saline group was the highest.Compared with the shamoperation group,the expressions of NF-κB in the other groups were obviously increased at different time（P〈0.01）.NF-κB in Huoxue Tongdu Decoction group was significantly lower than normal saline group（P〈0.05,P〈0.01）.At the same point,the expression of VCAM-1 in sham-operation group was the lowest and normal saline group was the highest.Compared with the sham-operation group,the expression of VCAM-1 in the other groups were obviously increased at different time（P〈0.01）.The observation in vascular expression rate： there was no positive vascular expression in sham-operation group and in three groups at 0.5 hour.But,positive vascular expression in normal saline group was significantly more than Huoxue Tongdu Decoction group at 1st,4h and 8h（P〈0.01）.Conclusion： Huoxue Tongdu Decoction could inhibit the expressions of NF-κB and VCAM-1 to alleviate the inflammatory reaction,which would help to reduce the ischemic-reperfusion injury of spinal cord.