旋覆代赭汤对反流性食管炎家兔食管黏膜脑肠肽和一氧化氮合酶的影响

Influence of Xuanfu Daizhe Decoction on brain-gut peptide and nitric oxide synthase in esophageal mucosa of rabbit with reflux esophagitis

目的:研究旋覆代赭汤对混合反流性食管炎(RE)家兔食管黏膜血管活性肠肽(VIP)、P物质(SP)和一氧化氮合酶(NOS2)水平的影响。方法:制备家兔RE模型,并进行常规HE染色观察食管黏膜病理改变,免疫组织化学技术检测兔食管黏膜VIP、SP、NOS2的水平。结果:与正常组比较,模型组家兔食管黏膜炎细胞浸润明显,VIP、NOS2表达明显升高(P<0.01),SP表达明显减弱(P<0.01);经中药治疗后全方组、甘升组、去苦降组、去升降相因组光镜下食管黏膜组织有不同程度的修复,其中全方组、甘升组食管黏膜中VIP的表达明显减弱,与模型组相比有显著性差异(P<0.01);与模型组比较,全方组、去升降相因组SP的表达显著增强(P<0.01);与模型组比较,全方组、去苦降组NOS2的表达显著减弱(P<0.01)。结论:旋覆代赭汤全方通过减少食管黏膜VIP和NOS2表达、增强SP表达调节胃肠运动,抑制胃肠内容物的反流和促进黏膜的修复,对RE治疗作用最好;而苦降组、升降相因组、去甘升组对RE治疗效果不佳。

Objective： To study the influence of Xuanfu Daizhe Decoction on vasoactive intestinal peptide（VIP）,substance p（SP） and nitric oxide synthase（NOS2） in esophageal mucosa of rabbit with reflux esophagitis.Methods： Rabbit models of reflux esophagitis were established,and esophageal mucosa pathological changes wer observed after HE staining.Immunohistochemical technique was used to test VIP,SP,and NOS2 levels in esophageal mucosa of rabbits.Results： Compared with normal group,the inflammatory cells infiltration in escophageal mucosa of rabbits in model group was significant,and expressions of VIP and NOS2 were increased significantly（P〈0.01）,while the expressions of SP was decreased significantly（P〈0.01）.After treatment of Chinese medicine,the escophageal mucosa of rabbits in Quanfang group,Gansheng group,Qukujiang group and Qushengjiang group were repaired to different degrees,and the expressions of VIP in escophageal mucosa of rabbits in Quanfang group and Gansheng group were weaken significantly compared with model group（P〈0.01）.Compared with rabbits in model group,the expressions of SP of rabbits in Quanfang group and Qushengjiang group were enhanced significantly（P〈0.01）,and the expressions of NOS2 of rabbits in Quanfang group and Qukujiang group were enhanced significantly（P〈0.01）.Conclusion： The whole prescription of Xuanfu Daizhe Decoction could treat reflux esophagitis effectively by decreasing the expression of VIP and NOS2,increasing the expressions of SP,adjusting gastrointestinal motility,inhibiting regurgitation of gastrointestinal contents,and promoting the restoration of mucosa,while the curative effect of Kujiang group and shengjiang group on reflux esophagitis was inefficacious.