利拉鲁肽对大鼠心肌缺血再灌注损伤的保护作用

Cardioprotective effects of liraglutidein rat myocardial ischemia-reperfusion injury studies

目的研究利拉鲁肽对大鼠心肌缺血再灌注损伤的保护作用与机制。方法将健康雄性的SD大鼠随机分为3组:假手术组、缺血再灌注组、利拉鲁肽组(70μg·kg-1),每组8只。结扎大鼠冠状动脉前降支30 min、开放120 min,制备心肌缺血再灌注模型。用伊文氏蓝、氯化三苯基四氮唑法测定心肌梗死面积;分别用原位末端标记法及免疫组化法检测心肌细胞凋亡及心肌凋亡蛋白Bcl-2、Bax蛋白的表达。结果利拉鲁肽组与缺血再灌注组相比,心肌梗死面积明显减小(均P<0.05);心肌细胞凋亡数明显减少,Bcl-2蛋白表达明显增高,Bax蛋白表达明显降低(均P<0.05)。结论利拉鲁肽可以通过抑制心肌细胞凋亡来减轻大鼠心肌缺血再灌注损伤。

Objective To investigate the effect and the mechanism of li-raglutideon myocardial ischemia reperfusion injury in rat.Methods Healthy male SD rats were randomly divided into three groups： shame-operated group , ischemia reperfusion （ I/R ） group, liraglutidegroup （ 70μg· kg -1 ） , 8 rats in each group.The rat model of myocardial I/R was established by occlusion of the left anterior descending coronary artery for 30 minutes and reperfusion for 120 minutes.The area at risk was deter-mined using Evans blue and infarct area using triphenyl tetrazolium chlo-ride staining.Apoptosis of myocardial cells was detected with in situ end labeling , and expressions of myocardial apoptosis proteins Bcl -2 and Bax were detected with immunohist ochemical method.Results In com-parison with ischemia reperfusion group , the myocardial infarction size and the number of apoptotic cells were reduced （ P〈0.05 ） , the expre-ssion of Bcl-2 protein was increased and Bax protein was decreased in liraglutide group （ P〈0.05 ）.Conclusion liraglutidecan protects cardio-myocytes from ischemia reperfusion injury by inhibiting cell apoptosis.