硅对干旱胁迫下四棱豆幼苗生物量和生理特性的影响

Effects of Silicon on Biomass and Physiological Properties of Winged Bean Seedling Under Drought Stress

采用营养液培养的方式,用PEG 6000（聚乙二醇6000,渗透势约为-0.315MPa）模拟干旱胁迫,研究了硅对干旱胁迫下四棱豆幼苗生物量和生理特性的影响。结果表明,与对照相比,干旱胁迫抑制了四棱豆幼苗生物量的积累,施用不同浓度的硅能有效缓解干旱胁迫对四棱豆幼苗生物量积累的抑制;与单独PEG 6000处理相比,模拟干旱胁迫下施硅处理提高了叶绿素含量、根系活力以及POD、CAD、SOD活性,降低了细胞膜透性、H2O2、脯氨酸和丙二醛含量以及脂氧合酶活性,降低了膜脂过氧化作用,缓解了干旱胁迫对细胞膜的伤害,增强了四棱豆幼苗的耐旱性,且1~1.5mmol/L的硅处理效果优于2mmol/L硅处理。

With solution culture method,the effects of exogenous silicon（Si）on biomass and physiological properties of winged bean seedling were investigated under drought stress simulated by PEG6000（osmotic potential was about-0.315MPa）.The results showed that drought stress significantly suppressed the biomass accumulation of winged bean,addition of different dose silicon could effectively relieve the repression of drought stress on the biomass accumulation of winged bean seedling.Compared with the plants treated with PEG alone,the treatment of silicon significantly improved Chlorophyll content,root activity,POD,CAD and SOD activity,reduced cell membrane permeability,H2O2,Pro and MDA content and LOX activity,decreased the lipid peroxidation of membrane,relieved the damage of drought stress on cell membrane and enhanced the drought resistance of winged bean seedling.The treatment with 1~1.5mmol/L silicon was more effective in improving the drought resistance of winged bean seedling than the treatment with 2mmol/L.