肿瘤组织库石蜡标本的制备及保存探讨

Study on the Paraffin Specimen Preparation and Preservation in Tumor Tissue Bank

近年来,随着对恶性肿瘤发病机制的研究进展加速,新方法增多,对肿瘤标本的需求量也越来越大,建立相应的肿瘤组织库(tumor tissue bank,TTB)势在必行。作为肿瘤标本的重要组成部分,高质量的石蜡标本具有珍贵的生物研究价值。与病理诊断不同,TTB的石蜡标本更注重其核酸和蛋白的质量控制。全文结合实际对TTB的石蜡标本制备和保存过程中存在的问题进行探讨,在传统方法的基础上进行改进,减少其DNA、RNA和蛋白质量的下降。

As the quick development of tumor study,better tumor specimen is in great demand.Scientists decide to establish Tumor tissue bank（TTB） which is believed be helpful improving specimen quality. Paraffin specimens is widly used in biological research,it is also the most important part of TTB. In this paper,the problems in paraffin specimens preparation and preservation were mainly discussed. And go through traditional technology and alterations recently,try to find a good way to keep DNA,RNA and protein stable in TTB.