维生素D及其受体对RIP1调控作用的研究

Study on the Regulation of RIP1 by Vitamin D and Vitamin D Receptor

目的探讨维生素D及其受体对受体相互作用蛋白(RIP)1表达的影响及其作用机制。方法采用实时定量PCR和Western blot技术,检测维生素D刺激对RIP1的m RNA和蛋白表达水平的影响,采用免疫共沉淀的方法探讨维生素D及其受体对RIP1的调节机制。结果维生素D及其受体能够显著降低RIP1的m RNA及蛋白水平表达,抑制HSP90与RIP1复合物的形成。结论维生素D及其受体通过调节HSP90与RIP1的相互作用调控RIP1的表达,为维生素D及其受体抑制肿瘤的发生提供了新的理论依据。

Objective Receptor Interacting Protein（RIP）is a crucial regulator in the signaling pathway of necrosis. This study aimed to investi-gate the effects of vitamin D and vitamin D receptor on the expression of RIP1 and to explore the underlying mechanism.MethodsThe expressionlevels of RIP1 m RNA and protein were detected by real-time quantitative PCR and Western blot. Then the co-immunoprecipitation assay was per-formed to detect the interaction between vitamin D,vitamin D receptor and RIP1.ResultsVitamin D and Vitamin D receptor significantly reducedthe transcription and expression levels of RIP1,and repressed the formation of the HSP90 and RIP1 complex.ConclusionVitamin D and vitaminD receptor could regulate the expression of RIP1 by regulating the interactions between HSP90 and RIP1,which provides a new theoretical basis forsuppression of the occurrence of tumors by vitamin D and its receptor.