pH对厌氧氨氧化反应脱氮效能的影响

Effect of pH on Denitrification Efficiency of Anaerobic Ammonia Oxidation Reaction

采用具有恒定pH功能的SBR接种厌氧氨氧化颗粒污泥,研究了pH对厌氧氨氧化菌脱氮效能的影响。实验结果表明:在进水ρ(氨氮)和ρ(亚硝酸盐氮)分别为58.00 mg/L和79.28 mg/L、TN容积负荷为0.82 kg/(m3·d)、不控制反应pH的条件下,随着反应的进行,pH不断升高,当pH=8.02时,菌种的去除效能最高;在进水ρ(氨氮)和ρ(亚硝酸盐氮)分别为120.00 mg/L和159.33 mg/L、TN容积负荷为1.62 kg/(m3·d)、恒定反应pH为8.00的条件下,反应4.0 h时的容积基质氮去除速率(NRR)达到1.42 kg/(m3·d),氨氮去除率达98.39%,亚硝酸盐氮去除率达99.48%;拟合曲线推导的最适pH的理论值为7.85,反应4.0 h时的NRR理论最大值达1.52 kg/(m3·d)。

The effect of pH on denitrifi cation effi ciency of anaerobic ammonia oxidation（ANAMMOX） bacteria was studied using ANAMMOX granular sludge in a SBR with constant pH. The experimental results show that： Under the conditions of infl uent ρ（NH4＋-N） and ρ（NO2--N） 58.00,79.28 mg/L respectively,TN volume loading 0.82 kg/（m3·d） and uncontrolled pH,the reaction pH increases continually and the denitrifi cation effi ciency of the bacteria is highest when pH=8.02;Under the conditions of influent ρ（NH4＋-N） and ρ（NO2--N） 120.00,159.33 mg/L respectively,TN volume loading 1.62 kg/（m3·d）,constant pH 8.00 and reaction time 4.0 h,the nitrogen removal rate（NRR）reaches 1.42 kg/（m3·d） and the removal rates of NH4＋-N and NO2--N are 98.39%,99.48% respectively;The fi tting curve shows that the theoretical optimum pH is 7.85,and the theoretical maximum NRR is 1.52 kg/（m3·d） at 4.0 h of the reaction time.