IFT80蛋白对肿瘤影响的研究

The effects of IFT80 proteins on tumors

目的:探讨IFT80(intraflagellar transport 80)蛋白在骨癌、肺癌、胰腺癌、胃癌、小肠癌、前列腺癌、乳腺癌和卵巢癌组织的表达分布情况与癌细胞增殖中的作用。方法:免疫组织化学研究IFT80的表达,免疫荧光和细胞培养研究抑制IFT80后对癌细胞增殖的影响和纤毛之间的关系。结果:1)IFT80在胃癌和肺癌组织中高表达,乳腺癌和小肠癌组织中中等表达,骨癌和卵巢癌组织中少量表达,前列腺癌和胰腺癌组织中几乎不表达;2)抑制IFT80后发现肺癌细胞增殖加快,纤毛减少变短;3)高分化,ⅡA期胃癌和正常胃组织中IFT80蛋白大量表达,而在低分化晚期,几乎不表达,在其他不同分化的胃癌中不同程度表达。结论:在不同癌组织中IFT80分布情况不一致。IFT80分布在细胞纤毛上,通过其表达的减少来调节纤毛的数量和长短参与癌细胞的增殖,故在严重癌组织中含量最低。

Objective： To investigate Intraflagellar Transport 80 （IFT80） protein expression in bone, lung, pancreatic, stomach, in- testinal, prostate, breast, and ovarian cancers to explore its mechanism in cancer cell proliferation and to diagnose and identify new tar- gets in cancer treatment. Methods： Immunohistochemistry was used to investigate the expression of IFT80 in gastric cancer tissue of different stages and in eight other kinds of human cancer tissues. We studied the relationship between cancer cell proliferation and inhi- bition of IFT80. Immunofluorescence method and cell culture were used to study the cilia and IFT80. Results： Results showed the fol- lowing： a） the expression of IFT80 was high in gastric and lung carcinoma tissues, moderate in breast and colorectal cancers, low in bone and ovarian cancers, and nearly absent in prostate and pancreatic cancers; b） inhibition of IFT80 in the A549 cancer cell line accel- erated cell proliferation and resulted in shorter, lower quality cilia; and c） IFT80 was abundantly expressed in cancer tissues of well-dif- ferentiated stage-IIA gastric cancer and normal gastric tissues, but was hardly expressed in late-stage, poorly differentiated gastric can- cer. IFT80 could have various degrees of expression in gastric carcinoma of other stages and differentiation. Conclusions： Different can- cer organs showed variation in IFT80 expression. IFTSO can be distributed in the organs with mechanical motion function, such as lungs and stomach. IFT80 is distributed on the cell cilia and can adjust the number and length of the cilia by reducing IFT80 protein ex- pression. Through a variety of ways, IFT80 directly or indirectly participates in the proliferation of cancer cells. Thus, the lowest or nearly zero expression of IFT80 can be seen in cancer tissues of high-grade malignancy, such as advanced cancers with poor differentia- tion.