硒化壳聚糖对K562/ADM细胞生物学行为的影响

Effect of Selenium Chiston on biological behaviour of human leukemia multidrug resistance cell line K562 / ADM in vitro

目的观察硒化壳聚糖对体外培养慢性粒细胞多药耐药白血病细胞株K562/阿霉素(ADM)细胞生物学行为的影响。方法硒化壳聚糖作用于体外培养的K562/ADM细胞12、24 h,应用流式细胞法检测细胞凋亡,软件拟合计算细胞周期;应用免疫印迹法检测P-糖蛋白(P-gp)的表达;应用RT-PCR法检测MDR-1 mRNA水平。结果硒化壳聚糖能够明显增强ADM对K562/ADM细胞的诱导凋亡作用,阻滞细胞周期于G1期(P<0.05,P<0.01),下调P-gp表达和MDR-1 mRNA水平(P<0.05,P<0.01),硒化壳聚糖浓度越高,作用效果越显著。结论硒化壳聚糖能够通过下调MDR-1基因和P-gp表达,阻滞细胞周期于G1期来诱导细胞凋亡,进而对体外培养的K562/ADM细胞耐药生物学行为产生逆转。

Objective To investigate the effect of Selenium chiston （Sc） on biological behaviour of human leukemia multidrug re-sistance cell line K562/ADM in vitro.Methods After treated with Sc for 12,24 h,the apoptosis rate and cell phase in K 562/ADM were ex-amined by flow cytometry.Expression of P glycoprotein （ P-gp） in K562/ADM was determined by Western blot and transcription level of MDR-1 gene was detected by semi-quantitative RT-PCR.Results Sc could obviously enhance the inducing effect of ADM on apoptosis of K562/ADM cell and block cell cycle into G1 phase（P〈0.05,P〈0.01）,down-regulate the P-gp and MDR-1 mRNA level in K562/ADM cell （ P〈0.05 ,P〈0.01 ） in a dose and time dependent manner .Conclusions Sc could change the biological behaviour of K 562/ADM cell in vitro through enhancing cell apoptosis ,blocking cell cycle in G1 phase,inhibiting the P-gp and MDR-1 gene expression.