寒冷应激对阿勒泰羊细胞免疫及热休克蛋白70mRNA表达的影响

Effects of Cold Stress on Immune System and hsp70 m RNA Expression in Altay Sheep

为研究不同的寒冷应激温度对阿勒泰羊(Ovis aries)细胞免疫及热休克蛋白70的影响。实验采集阿勒泰羊寒冷应激前后肝、肺、脾、淋巴结组织及血清,采用实时荧光定量PCR中最大二阶导数法(2-ΔΔCt)对各组织中hsp70的表达量进行统计学分析,同时采用ELISA方法测定血清中白细胞介素-4(IL-4)及白细胞介素-2(IL-2)寒冷应激前后浓度变化。结果显示,在寒冷条件下阿勒泰羊各种组织中热休克蛋白hsp70的表达都有所增加,尤其是脾组织的表达增加幅度较大。ELISA方法测定阿勒泰羊在寒冷刺激后IL-4浓度发生显著下降(P=0.016),而IL-2在冷应激后变化不显著(P=0.502),出现轻微的下调。研究表明,寒冷应激条件下,较高水平的hsp70能够保护机体免受应激的损伤。而在冷应激过程中,机体的免疫系统受到抑制。

The effects of old stress at different temperatures on the cellular immunity and heat shock protein 70 expression were studied in Altay sheep （Ovis aries）. Collected before and after the test cold stress Altay sheep liver, lung, spleen, lymph node tissue and serum, and expression of hsp70 in each tissue was examined using real-time quantitative PCR, and the change of interleukin -4 （IL-4） and interleukin -2 （IL-2） concentration in the serum was tested using ELISA method. The results showed that the expression of hsp70 expression increased in various tissues after cold cold stress, with the highest increase in spleen tissue. The concentration of IL-4 decreased significantly after cold stimulation （P = 0.016）, while IL-2 did not change significantly after cold stress （P = 0.502）, but a slight reduction. Studies have shown that, under conditions of cold stress, a high level of hsp70 expression protects the body from injury, and that the body＇s immune system is suppressed.