摘要
目的建立动物心脏缺血再灌注模型,探讨可溶性糖基化终末产物受体(sRAGE)对缺血再灌注诱导的心功能及心肌细胞凋亡的作用。方法复制C57BL/6J小鼠心脏缺血再灌注模型,利用超声检测心功能;通过TUNEL染色及Caspase-3活性检测,评价心肌细胞凋亡的程度。结果与sham组比较,I/R组左室射血分数降低[sham组为(72.4±2.1)%,I/R组为(30.9±3.2)%,P<0.05],短轴缩短率降低[sham组为(40.7±1.6)%,I/R组为(15.1±2.0)%,P<0.05],TUNEL阳性心肌细胞数目增加[sham组为(1.0±0.2)%,I/R组为(20.0±1.6)%,P<0.05],Caspase-3活性升高[(sham组(1.00±0.2)%比I/R组(2.64±0.4)%,P<0.05)。与I/R组相比较,sRAGE预处理能够明显升高左室射血分数[(46.5±2.0)%P<0.05],增加短轴缩短率[(23.0±1.1)%,P<0.05],同时降低TUNEL阳性心肌细胞数目[(9.2%±1.0)%P<0.05],和Caspase-3活性[(1.94%±0.1)%P<0.05]。结论 sRAGE能够明显改善缺血再灌注诱导的心功能降低,并抑制心肌细胞的凋亡。
Objective To test the effect of sRAGE on cardiac function and myocardial apoptosis induced by ischemia-reperfusion in vivo. Methods C57 BL /6J mice with the ligation of left anterior descending coronary artery were used as the in vivo model. At the end of reperfusion,cardiac function was evaluated with echocardiography,and myocardial apoptosis was detected by TUNEL staining and caspase-3 activaty. Results Compared to the sham group,I / R decreased left ventricular ejection fractions( EF) [( 30. 9 ± 3. 2) % vs( 72. 4 ± 2. 1) %,P〈0. 05 ],and left ventricular fractional shortening( FS) [( 15. 1 ± 2. 0) % vs( 40. 7 ± 1. 6) %,P〈0. 05],and then increased TUNEL [( 20. 0 ± 1. 6) % vs( 1. 0 ±0. 2) %,P〈0. 05],and Caspase-3 activaty [( 2. 64 ± 0. 4) % vs( 1. 00 ± 0. 2) %,P〈0. 05]. Compared to I / R group,sRAGE pretreatment significantly improved EF [( 46. 5 ± 2. 0) % vs( 30. 9 ± 3. 2) %,P〈0. 05],and FS[( 23. 0 ± 1. 1) %vs( 15. 1 ± 2. 0) %,P〈0. 05 ],decreased TUNEL [( 9. 2 ± 1. 0) % vs( 20. 0 ± 1. 6) %,P〈0. 05 ],and Caspase-3activaty[( 1. 94 ± 0. 1) % vs( 2. 64 ± 0. 4) %,P〈0. 05]. Conclusion sRAGE inhibits cardiac dysfunction and myocardial apoptosis induced by ischemia-reperfusion in vivo.
出处
《解剖学报》
CAS
CSCD
北大核心
2015年第2期202-207,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(81370313
30801217)
北京市科技新星资助项目(2010B050)
北京市卫生系统高层次卫生技术人才资助项目(2013-3-046)
关键词
可溶性糖基化终末产物受体
缺血再灌注
心功能
心肌细胞
原位缺口末端标记
小鼠
Soluble form of receptor for advanced glycation end product
Ischemia-reperfusion
Cardiac function
Myocardium
Terminal UTP nick end labeling
Mouse