摘要
目的探讨膜联蛋白A7(ANXA7)低表达对人胃癌低分化细胞MGC-803的影响。方法将细胞分为实验组、阴性对照组和空白对照组。采用RNA干扰技术将靶向ANXA7的siRNA和阴性对照siRNA以脂质体转染法分别转染胃癌细胞系MGC-803细胞,空白对照组不予任何处理。转染48h后,采用Western blotting和实时定量-PCR法进行抑制效果的鉴定。检测ANXA7低表达对MGC-803细胞黏附、伸展、剥脱和迁移等生物学行为的影响。结果靶向ANXA7的siRNA可显著抑制ANXA7的表达;ANXA7表达受抑后,细胞黏附、伸展和剥脱能力降低,与阴性对照组和空白对照组相比,差异显著(P<0.05)。细胞迁移变化不显著。结论 ANXA7表达降低后MGC-803细胞行为学发生明显改变。
Objective To explore the effect of annexin A7（ ANXA7） knockdown on biological behaviour of MGC-803 cells. Methods The MGC-803 cells were grouped into experiment group,negative group and blank group. RNA interference technology was used to transfect the siRNA targeted ANXA7,scrambled siRNA into MGC-803 cells with lipofectine 2000 without any treatment to the blank group. After transfection for about 48 hours,Western blotting and Realtime PCR were used to assure the suppression of ANXA7 on both protein level and mRNA level. Cell adhesion assay,detachment assay,and spreading assay and wound healing assay were used to detect the adhesion,detachment,spreading and migration abilities of MGC-803 cells. Results The expression of ANXA7 was significantly suppressed in the cells which were transfected with ANXA7 siRNA. When ANXA7 expression was suppressed,the cell adhesion rate,detachment rate,spreading rate in experiment group were significantly lower than those in negative group and blank group（ P〈0. 05）.Compared with the negative group and blank group,migration ability of experiment group was not significantly different.Conclusion The biological behaviour of MGC-803 cells change obviously when ANXA7 expression is suppressed and ANXA7 could become potential target of therapeutic strategies.
出处
《解剖学报》
CAS
CSCD
北大核心
2015年第2期216-220,共5页
Acta Anatomica Sinica
基金
河北省高校省级重点学科资助项目[冀教高(2003)4号]
