血清Talin-1在原发性肝细胞癌早期诊断中的作用

Effect of serum Talin-1 in early diagnosis of hepatocellular carcinoma

目的研究原发性肝细胞癌(hepatoce lloular carcinoma,HCC)患者血清中的踝蛋白1(Talin-1),并与传统的生物学标志物甲胎蛋白(alpha-fetoprotein,AFP)相比,探讨其在HCC早期诊断中的作用。方法采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)分别检测120例血清样本中的Talin-1,包括HCC血清40例、肝硬化(liver cirrhosis,LC)血清40例、健康对照组血清40例。结果用ROC曲线创建一个Talin-1相对于AFP的诊断预测模型。HCC组Talin-1血清中的表达水平高于其他组(P<0.05)。在HCC的诊断中,Talin-1诊断的准确性明显高于AFP相关的敏感性、特异性。结论 Talin-1在HCC的早期诊断中可能是一个潜在的标志物,并具有比传统的生物学标志物AFP更高的敏感性和特异性。

Objective To investigate serum Talin-1 level in hepatocellular carcinoma （HCC） patients and to access the diagnostic value of serum Talin-1 compared to the most feasible traditional biomarker alpha-fetoprotein （AFP） for the diagnosis of HCC. Methods Talin-1 was detected by enzyme linked immunosorbent assay （ELISA） in serum samples from 120 subjects including 40 cases with HCC, 40 cases with liver cirrhosis （LC） and 40 cases healthy controls （HC）. Results ROC curve analysis was used to create a predictive model for Talin-1 relative to AFP in HCC diagno- sis. Serum levels of Talin-1 in HCC patients were significantly higher compared with other groups （P 〈 0.05）. The di- agnostic accuracy of Talin-1 was higher than that of AFP regarding sensitivity, specificityin diagnosis of HCC. Conclu- sion Talin-1 maybe a potential novel biomarker for the diagnosis of HCC.