细胞周期依赖性蛋白激酶1(Cdk1/p34cdc2)对喉癌术后局部复发的预测价值

Predicted value of cell Cycle-dependent protein kinase 1(Cdk1/p34cdc2) for local recurrence of laryngeal cancer

目的检测喉鳞状细胞癌及癌旁切缘组织中p53、p21和Cdk1/p34cdc2蛋白表达及其与患者预后的关系。方法应用免疫组化SP法检测85例喉鳞状细胞癌及癌旁切缘组织中p53、p21和Cdk1/p34cdc2蛋白的表达,并收集临床病理资料进行随访。结果在随访2年以上的喉鳞状细胞癌中14例出现局部复发(复发组),71例未复发(未复发组)。在喉鳞状细胞癌和癌切缘组织中p53蛋白阳性率分别为60.0%和36.5%;p21蛋白阳性率分别为38.8%和21.2%;Cdk1/p34cdc2蛋白阳性率分别为70.6%和29.4%。喉鳞状细胞癌复发组和未复发组的切缘组织中p53蛋白阳性率分别为71.4%和29.6%(P=0.003),切缘组织中p21蛋白阳性率分别为50.0%和15.5%(P=0.004),切缘组织中Cdk1/p34cdc2蛋白阳性率分别为57.1%和23.9%(P=0.013)。癌旁切缘组织中p53与p21及Cdk1/p34cdc2蛋白表达之间无相关性(P均>0.05)。结论 p53、p21和Cdk1/p34cdc2蛋白在喉鳞状细胞癌的发生、发展中可能起重要作用;癌旁切缘组织中p53、p21和Cdk1/p34cdc2过表达与喉鳞状细胞癌局部复发密切相关。

Purpose To study the expression of p53, p21 and Cdkl/p34cdc2 in the laryngeal cancer and its margin tissues and to ex- plore their relationship with local recurrence of laryngeal cancer. Methods A total of 85 patients with early laryngeal cancer were se- lected randomly during 2004 to 2010 in Tangshan Union Hospital, Hebei, China. SP immunohistochemical method was used to detect the expression of p53, p21 and Cdkl/p34cdc2 in the tumor and margin tissues. Pathological data were collected for follow-up. Results In more than 2 years of follow-up study, 14 of 85 patients with laryngeal cancer presented with recurrence （recurrent group） , while 71 patients without recurrence （ none recurrent group）. The positive rate of p53 protein in laryngeal cancer and its margin tissues was 60. 0% and 36. 5% , respectively, the positive rate of p21 protein in laryngeal cancer and its margin tissues was 38.8% and 21.2% , respectively. The positive rate of Cdkl/p34cdc2 in laryngeal cancer and its margin tissues was 70. 6% and 29.4% , respectively, p53 protein in the surgical margin of the recurrent group and non recurrent group was 71.4% and 29.6% （P = 0. 003 ）, that of p21 was 50. 0% and 15.5% , （P =0. 004） and Cdk1/p34cdc2 was 57.1% and 23.9% （P =0. 013）, respectively. There was no correlation between expression of p53 with p21 protein and Cdkl/p34cdc2 protein（P 〉 0. 05）. Conclusion p53, p21 and Cdkl/p34cdc2 may be involved in the occurrence, development and recurrence of laryngeal squamous cell carcinoma. Overexpression of p53, p21 and Cdkl/p34Cdc2 in the surgical margin is closely related to local recurrence of laryngeal cancer.