人毛滴虫膜性细胞器的电镜细胞化学研究

ELECTRON MICROSCOPIC CYTOCHEMISTRY STUDIES ON MEMBRANE-BOUND ORGANELLES OF TRICHOMONAS HOMINIS

本文应用电镜细胞化学技术,对人毛滴虫膜性细胞器进行研究,结果表明:酸性磷酸酶与胞嘧啶核苷酸酶反应产物均定位于初级溶酶体、消化泡和副基体成熟面囊泡;硫胺素焦磷酸酶和烟酞胺腺瞟呤二核苷酸酶反应分别显示于副基体成熟面膜囊与中间胶囊;消化泡中可见过氧化物酶反应物;而细胞色素氧化酶与过氧化氢酶反应则为阴性,提示虫体缺乏线粒体与微体。铀-铅-铜浸染法可选择性深染氢化酶体、副基体和内质网。对细胞化学反应结合膜性细胞器功能进行了讨论。

This paper presents the results of an electron microscopic cytochemistry study on the menbrane-bound organelles of Trichomonas hominis. Acid phosphatase (ACP) and cyti-dine monophosphatase (CMPase) were located in the primary lysosomes, digestive vacuales, as well as in the parabasal body stacks, being usually in its mature surface sacs. Thiamine pyrophosphatase (TPPase) and nicotinamide adenine dinucleotide phosphatase (NADPase) were found in the saccules, being on the mature surface and in the intermediate saccules of parabasal body stacks, respectively. The reactive products of peroxidase (POase) were seen only in the digestive vacuoles. Cytochrome oxidase (COase) and catalase (Cat) reactions were both negative. It is suggested that T. hominis lacks mitochondrion and microbody but possesses hydrogenosome. The parabasal body, endoplasmic reticulum and hydrogeno-some were stained well with the U-Pb-Cu impregnation technique. (Figsl-11)