摘要
目的观察血管紧张素Ⅱ(AngⅡ)与转化生长因子-β1(TGF-β1)在顺铂肾损伤中的作用及二者的相关性。方法取16只大鼠随机分为正常组和模型组,各8只。采用尾静脉注射的方法复制顺铂肾损伤模型,6周后以放射免疫法检测肾组织中AngⅡ水平,免疫组化法观察TGF-β1在肾组织中的表达。结果与正常组比较,模型组肾组织中AngⅡ含量明显增加,TGF-β1在肾小管上皮细胞的表达明显增强(P<0.01),且二者之间有一定相关性(Y=0.138 X+0.062 8,r=0.98)。结论肾脏局部AngⅡ的水平与TGF-β1的表达可能在顺铂肾损伤的发生、发展中起一定作用,且二者呈正相关。
Objective To explore the role of angiotensin（AngⅡ） and transforming growth factor-β1 （TGF-β1） on cisplatin induced renal injury and their correlation.Methods 16 rats were randomly divided into the two groups,normal group and model group.The caudal vein injection was adopted to duplicate the cisplatin induced renal injury model in rat.The level of Ang Ⅱ in the renal tissue after 6 weeks was detected by radioimmunoassay and the expression of TGF-β1 in the renal tissue was observed by the immunohistochem-istry method.Results Compared with the blank group,the level of Ang Ⅱ in the model group was significantly increased and the ex-pression of TGF-β1 in the tubular epithelia was obviously enhanced（P〈0.01）,moreover certain correlation existed between them（Y=0.138X＋0.062 8,r=0.98）.Conclusion The AngⅡ level and TGF-β1 expression may play a certain role in the occurrence and development of cisplatin induced renal injury and show the positive correlation.
出处
《中国药业》
CAS
2015年第7期34-35,共2页
China Pharmaceuticals
