摘要
目的建立测定血安胶囊中没食子酸含量的反相高效液相色谱法。方法色谱柱采用Hypersil ODS2柱（200mm×4.6mm,5μm）,流动相为甲醇-0.1%磷酸溶液（3∶97）,检测波长为214nm,流速为1.0mL/min。结果没食子酸进样量在0.0211-0.211μg范围内与峰面积呈良好线性关系（R2=1）,平均回收率为99.28%,RSD=1.73%（n=6）。结论该方法简便、快速、结果准确、重复性好,可用于血安胶囊中没食子酸含量的测定。
Objective To establish a RT-HPLC method for determining the gallic acid content in Xuean Capsule by RP-HPLC.Methods The octadecyl silane bonded silica gel column ODS-C18 was adopted with the mixture solvent of methanol-0.1% phosphoric acid（3 ∶97） as the mobile phase,the flow rate was 1.0 m L/min and the detecting wavelength was 214nm.Results The sample size of gallic acid in the range of 0.021 1-0.211 μg/m L showed the good linear relation with the peak area.The average recovery rate was 99.28%,RSD=1.73%（n=6）.Conclusion It is concluded that with the parameter set used in this study,the gallic acid content in Xuean capsules can be determined in a fast and accurate manner with a good reproducibility.
出处
《中国药业》
CAS
2015年第7期51-52,共2页
China Pharmaceuticals
