污水处理厂耐四环素乳糖发酵型肠杆菌及四环素抗性基因的研究

Study on Tetracycline- resistant Lactose- fermenting Enterobacteriaceae and Tetracycline Resistance Genes in Sewage Treatment Plant

采用传统PCR法分析了不同季节城市污水处理厂进水、活性污泥和出水中耐四环素乳糖发酵型肠杆菌（TR-LFE）和乳糖发酵型肠杆菌（LFE）所含四环素耐药基因（tet-R基因）的多样性和数量。结果表明,在分离得到的528株TR-LFE和LFE中含有tet（A）、tet（B）、tet（C）、tet（D）、tet（E）、tet（M）、tet（G）和tet（X）等tet-R基因,检出比例为0~85.71%不等。

In this paper,traditional method of qualitative PCR was applied to detect the diversity and abun-dance of tetracycline resistance genes（tet-R gene）in the tetracycline-resistant lactose-fermenting Enterobac-teriaceae（TR-LFE）and lactose-fermenting Enterobacteriaceae（LFE）separated from influent,sludge and efflu-ent of city sewage treatment plant in different seasons. The result shoued among the 528 LFE strains,tet-R genes such as tet（A）、tet（B）、tet（C）、tet（D）、tet（E）、tet（M）、tet（G）and tet（X）were detected,the rates ofdetection were 0~85.71%.