猪伪狂犬病毒YZ株gE全基因的克隆与序列分析

Cloning and Sequence Analysis of the Full-lengthg E Gene of Pseudorabies Virus YZ Strain

为了解猪伪狂犬病毒（PRV）gE基因的遗传变异特性,根据Gen Bank已发表的PRV gE全基因序列,设计并合成1对特异性引物,对PRV YZ株进行了gE全基因的扩增、克隆及测序,并与其他参考毒株gE基因进行比较序列分析。测序结果表明,YZ株gE全基因序列由1862bp组成,与GenBank已发表的13株PRV gE参考株序列同源性介于97.9%-99.9%。进化树分析表明,YZ株与目前国内流行的毒株在同一进化分支内,与国外分离株有一定差异。

In order to investigate the genetic mutation features of PRV gE,one pair of primers was designed and synthesized to amplify the full-length gE gene of PRV YZ strain according to the genomic sequence of PRV gE published in Gen Bank.PRV gE gene was amplified by PCR.The purified PCR products were cloned into p MD18–T vector and sequenced.The sequencing results showed that the full-length gE gene of PRV YZ consisted of 1 862 nucleotides,the homologies were 97.9%-99.9% when compared with gE sequences of 13 PRV published in Gen Bank.Phylogenetic analysis showed that PRV YZ isolate had a closer relationship with other domestic isolates than foreign reference strains.