暴马丁香不同部位乙醇提取物抑菌及清除DPPH自由基作用

Anti-bateria and DPPH radical scavenging activity of different parts from Syringa Reticulata( Blume) Hara var.

目的以暴马丁香树皮、叶、枝和花蕾的75%乙醇提取物为材料,研究其抑菌及抗氧化的活性。方法采用滤纸片法测定暴马丁香不同部位乙醇提取物对2种常见细菌的生长抑制活性、最低抑菌浓度。采用DPPH自由基法,利用分光光度法测定不同部位乙醇提取物在不同质量浓度下清除DPPH自由基的能力。结果暴马丁香乙醇提取液对沙门氏菌的抑制效果大小顺序为枝>花蕾>叶>树皮,对枯草芽孢杆菌的抑制效果大小为叶>枝>树皮>花蕾。暴马丁香树皮乙醇提取物抗氧化能力最强,其余各部位的抗氧化能力大小依次是花蕾>枝>叶。结论暴马丁香枝和叶的抑菌能力较好,其树皮、花蕾的抗氧化能力较强,确定枝和花蕾具有较好的应用价值。

Objective The context was performed to investigate the antioxidant activity and anti-bacteria of the 75%（ v / v） ethanol extraction of different parts from Syringa Rticulate（ Bl.） Hara var. mandshurica（ Maxim.） Hara. Methods The growth inhibitory activity and the lowest concentration for anti-bacteria of the two common bacteria were determined by filter paper method. And the DPPH radical-scavenging activity with different concentrations of different parts from the herb was determined by spectrophotometry method. Results The order of inhibitory effect of the ethanol extraction of the Syringa Reticulata（ Blume） Hara var. on salmonella is branch 〉 flower 〉 leaves 〉 bark. The order of the inhibitory effect on bacillus subtilis is leaves 〉 branch 〉 bark 〉 flower. The bark of the plant has the strongest scavenging activity for DPPH radical,which was better than flower,branch and leaves in order. Conclusion The branch and leaves of Syringa Reticulata（ Blume） Hara var. possess the strongest antibacterial activity,while its bark and flower possess a strong antioxidant activity. As a result,the branch and flower of the plant possess better application value.