肺腺癌人转录辅助因子4过表达与淋巴转移的相关性

Correlation between overexpression of PC4 in lung adenocarcinoma with lymph node metastasis

目的探讨肺腺癌组织中人转录辅助因子4(PC4)过表达对淋巴转移的促进作用。方法选取96份肺腺癌组织标本,采用免疫组织化学法和荧光定量PCR法检测PC4蛋白和mRNA的表达水平,并分析其与淋巴转移和肿瘤分期的相关性。结果肺腺癌组织PC4蛋白和mRNA表达明显正相关(r=0.63,P<0.01);PC4蛋白表达与淋巴转移正相关(χ2=8.29,P<0.01),与TNM分期正相关(χ2=4.71,P<0.05);PC4 mRNA与淋巴转移正相关(χ2=8.40,P<0.01),与TNM分期正相关(χ2=5.10,P<0.05)。结论 PC4过表达与肺腺癌患者的淋巴转移、TNM分期密切相关,PC4可能促进肺腺癌的淋巴转移。

Objective To investigate the promotion effect of human transcriptional positive cofactor 4 （PC4） overexpression on lymphatic metastasis in lung adenocarcinoma .Methods 96 samples of lung adenocarcinoma tissue were collected .The immuno‐histochemistry（IHC） and real‐time quantitative polymerase chain reaction （qRT‐PCR） were adopted for detecting the expression levels of PC4 protein and mRNA .The correlation of PC4 expression with lymphatic metastasis and TNM stage was analyzed .Re‐sults The expression of PC4 protein was positively correlated mRNA in lung adenocarcinoma （r=0 .63 ,P〈0 .01）;the expression of PC4 protein was positively correlated with lymph node metastasis （χ2 =8 .29 ,P〈0 .01） and TNM stage （χ2 =4 .71 ,P〈0 .05）;the expression of PC4 mRNA was also positively correlated with lymph node metastasis （χ2 = 8 .40 ,P〈 0 .01） and TNM stage （χ2 =5 .10 ,P〈0 .05） .Conclusion PC4 overexpression is found to be closely associated with the lymph node metastasis and TNM stage .PC4 may facilitate the lymph node metastasis of lung adenocarcinoma .