UNC5C基因启动子的甲基化状况与大肠癌UNC5C的相关性分析

Correlation analysis of methylation status of UNC5C gene promoter with colorectal cancer UNC5C

目的探讨UNC5C基因启动子的甲基化状况与大肠癌UNC5C的相关性。方法选择河北省唐山市开滦总医院肿瘤科2010年2月至2013年3月共54例散发性大肠癌及相关正常黏膜组织,以及6个大肠癌细胞株和纤维细胞株（FCL）作为研究对象。对研究对象实施基因mRNA和甲基化分析。结果 UNC5A和UNC5B的mRNA,在所检测的大肠癌细胞株中均有表达。而UNC5C除FCL外,所有的癌细胞株中均无mRNA表达。在大肠癌组织中UNC5C的甲基化明显高于正常黏膜,差异有统计学意义（P〈0.05）。UNC5C基因在Ⅰ~Ⅱ期,以及Ⅲ~Ⅳ期的甲基化水平均显著高于未甲基化水平,均差异有统计学意义（P〈0.05）。依照Spearman法进行相关性分析,UNC5C缺陷与大肠癌疾病呈正相关（r=0.856,P〈0.05）。结论 Netrin-1受体UNC5C缺陷与大肠癌具有一定相关性。

Objective To investigate the correlation between methylation status of UNC 5C gene promoter with colorectal cancer UNC5C .Methods 54 cases of sporadic colorectal cancer and related normal mucosal tissue ,as well as 6 colon cancer cell lines and fiber cell lines（FCL） in the oncology department of the Kailuan General Hospital from February 2010 to March 2013 were selected as the research objects and performed the mRNA and methylation analysis for exploring the correlation between the netrin‐1 receptor UNC5C defects with colorectal cancer disease .Results mRNA of UNC5A and UNC5B was expressed in the detected colorectal cancer cell lines .Except FCL for UNC5C ,all of the cancer cell lines had no mRNA expression .In colorectal cancer tissue , UNC5C methylation was significantly higher than that in the normal mucosa ,the difference was statistically significant （P〈0 .05） . The methylation levels of UNC5C gene in the stage Ⅰ to Ⅱ ,and the stage Ⅲ to Ⅳwere significantly higher than non‐methylation levels ,the differences were statistically significant （P〈0 .05） .The correlation analysis by the Spearman method showed that the UNC5C defects was positively correlated with colorectal cancer disease （r= 0 .856 ,P〈 0 .05） .Conclusion Netrin‐1 receptor UNC5C defect has certain correlation with colorectal cancer disease .