玻璃器皿盛装口腔小器械灭菌开封后有效期的研究

Investigation on validity period of glassware containing sterilized oral small instruments after disinfection unsealing

目的探讨玻璃器皿盛装口腔小器械灭菌开封后的有效期。方法采用方便抽样的方法在正常工作日选取口腔诊室15间,每间诊室选取已灭菌玻璃器皿(内盛灭菌小器械)2个,在玻璃器皿开封后使用过程中的4、8、24、48、72、96、120、144、168h采集样本进行培养,观察细菌生长情况。结果 30个玻璃器皿,在开封使用后的4、8、24、48h均无细菌生长;第72h的采样培养中有3个细菌生长,与4、8、24、48h比较差异无统计学意义(P>0.05);在96h时的采样培养中共有10个细菌生长,与4、8、24、48h比较差异有统计学意义(P<0.05)。玻璃器皿盛装口腔小器械灭菌开封后的有效期可为48h。结论玻璃器皿盛装口腔小器械灭菌开封后的有效期可为48h,有效期的延长可减少器械的灭菌次数,降低器械的耗损和延长器械使用寿命。

Objective To study the validity period of glassware containing sterilized oral instruments after disinfection unsea‐ling .Methods Fifteen stomatology consulting rooms were selected on the normal working day by using the convenience sampling method .2 sterilized glass containers （filled with sterilized instruments） were selected in each room .The samples were collected at 4 ,8 ,24 ,48 ,72 ,96 ,120 ,144 ,168 h during the use process after unsealing the containers and the growth situation of bacteria was carefully observed .Results Thirty glasswares had no bacterial growth at 4 ,8 ,24 ,48 h during the use process after unsealing ,only 3 bacteria grew in the sampling culture at 72 h ,the differences had no statistical significance compared with at 4 ,8 ,24 ,48 h （P＆gt;0 .05）;10 bacteria grew in the sampling culture at 96 h ,the differences had statistical significance compared with at 4 ,8 ,24 ,48 h （P〈0 .05） .The validity period of glassware containing sterilized oral instruments after disinfection unsealing was 48 h .Conclusion The validity period of glassware containing sterilized oral instruments is 48 h after disinfection unsealing ,the extension of validity period can decrease the sterilization times ,reduce the equipment wastage and prolong the equipment service life .