广西汉族IL-10启动子区基因多态性与HBV易感性及感染转归的关系

Relationship of interleukin-10 gene promoter polymorphism with susceptibility of hepatitis B virus infection and its outcome in Guangxi Han population

目的探讨白细胞介素-10(IL-10)基因启动子区-592A/C、-1082G/A位点单核苷酸多态性与乙型肝炎病毒(HBV)易感性及后临床转归之间的关系。方法 256例慢性HBV感染者,其中乙型肝炎肝硬化59例,慢性乙型肝炎151例,慢性HBV携带46例,以52例健康体检者作为对照组。采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术,检测各组IL-10基因启动子区-592A/C、-1082G/A位点基因型及等位基因分布频率。结果肝硬化组、肝炎组、携带组与对照组的IL-10-592A/C位点AA、AC、CC基因型及A、C等位基因分布频率比较,差异均无统计学意义(P>0.05);IL-10-1082G/A位点GG、GA和AA基因型分布频率比较,差异有统计学意义(P<0.05),AA基因型频率分布依次为肝硬化组>肝炎组>携带组>对照组(P<0.05);各组IL-10-1082G/A位点G、A等位基因频率比较,差异有统计学意义(P<0.05),肝硬化组的A等位基因频率分布最高,其余依次为肝炎组、携带组及健康对照组(P<0.05)。不同HBV-DNA水平组的IL-10-592A/C、IL-10-1082G/A两位点基因型及等位基因分布频率比较,差异均无统计学意义(P>0.05)。结论IL-10-1082G/A位点AA基因型及A等位基因可能与广西汉族人群HBV易感性及其临床结局有关;而IL-10基因启动子区-592A/C、-1082G/A位点多态性可能与广西汉族人群感染HBV后的病毒复制水平无明显关系。

Objective To explore the relationship of single nucleotide polymorphism （ SNP ） of interleukin-10 （ IL-10 ） gene promoter-592A/C and -1082G/A with susceptibility of hepatitis B virus （HBV） infection and its clinical outcome .Methods Polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP） assay was used to determine the distribution frequencies of genotype and allele of IL-10-592A/C and -1082G/A among 256 cases of chronic HBV infection,including 59 cases of liver cirrhosis（LC）,151 cases of chronic hepatitis B（CHB） and 46 cases of chronic asymptomatic HBV carrier （ASC）,as well as among 52 healthy controls. Results There was no significant difference in genotype AA , AC and CC frequencies of IL-10-592A/C in each chronic HBV group compared with healthy controls,as well as allele A and C frequencies（P＆gt;0.05）.There was significant difference in genotype GG ,GA and AA frequencies of IL-10-1082G/A in each chronic HBV group compared with healthy controls,as well as allele G and A frequencies（P＆lt;0.05）, and the genotype AA and allele A frequencies of IL-10-1082G/A were significantly higher in LC patients than those in CHB patients,ASC patients and healthy controls（ P＆lt;0.05）.No significant difference was found in frequencies of genotypes and alleles of IL-10-592 A/C and IL-10-1082G/A between HBV-DNA＆lt;1.0 ＆#215;103 copies/ml group and HBV-DNA ＆gt;1.0 ＆#215;103 copies/ml group（P ＆gt;0.05）.Conclusion IL-10-1082G/A genotype AA and allele A might be associated with the susceptibility of HBV infection and its clinical outcome in Guangxi Han population.The gene polymorphisms of IL-10 promoter-592A/C and-1082G/A might not be significantly associated with HBV-DNA replication after being infected with HBV in Guangxi Han population.