怡乐村并殖吸虫和卫氏并殖吸虫同工酶等电聚焦的研究

STUDY ON ISOENZYMES OF PARAGONIMVSILOKTSUENENSIS AND PARAGONIMUSWESTERMANI BY ISOELECTRIC FOCUSING

采用薄层等电聚焦和聚丙烯酰胺凝胶电泳方法对我国东北地区怡乐村并殖吸虫和卫氏并殖吸虫6种同工酶的比较研究结果表明:两者同工酶表现出各自的种特征性酶谱,其中以单虫样品分析的苹果酸脱氢酶在两者均存在种群内多态现象,依据酶谱可以对两虫种作出区分。结果提示它们的遗传结构存在明显不同。因而认为怡乐村并殖吸虫是不同于卫氏并殖吸虫的独立虫种。

The isoenzyme patterns of Paragonimus iloktsuenensis and P. westermani were studied by isoelectric focusing on polyacrylamide gels for PGM, MDH, HK, ME, PO and ACP. Interspecific differences were apparent as shown by the isoenzyme profiles of these enzymes examined. MDH was polymorphic with 2 recognizable phenotypes, designated common type and variant type respectively, in P. L and with 3 phenotypes, designated MDH1 ,MDH2 and MDH1-2 respectively, in P. w. The result showed that the two species could be distinguished one from another by such species-specific band patterns of isoenzymes. This suggests that P. w. and P. i. have undergone geneticaly divergent development and P. i. can be regarded as a valid species different from P. w. In the light of MDH polymorphism, some implications of isoenzyme analysis on taxonomy of the genus Paragonimus were discussed (Figs. 1-6).