基于鲁米诺-过氧化氢-CdTe的流动注射-化学发光体系及应用于多巴胺的检测

Determination of dopamine with the enhanced chemiluminescence system of luminol-H_2O_2 in the prescence of CdTe

在碱性介质中,CdTe量子点对鲁米诺-过氧化氢体系有增敏作用,而多巴胺对该体系有着抑制作用。结合流动注射化学发光技术,建立了快速测定多巴胺的流动注射化学发光分析法,并对可能的机理进行了探讨。结果表明,在优化条件下,多巴胺在1.2×10^-5-7.8×10^-8mol/L浓度范围与化学发光强度呈良好的线性关系,检测限为1.5×10^-9mol/L,对于浓度为5×10^-6mol/L的多巴胺,平行测定11次的相对标准偏差为2.0%.此法成功用于尿液和制剂中多巴胺的测定。

In alkaline media, CdTe quantum dots can gzeafly enhance the chemiluminescence between ]umlnol and hydrogen peroxide, however, dopamine hydrechloride can inhibit the CL of luminol- H2O2 -CdTe QDs system. Based on this characteristic, a simple and convenient method for the determination of dopamine hydrochloride was developed by combination chemiluminescence and flow - injection analysis technique. The possible CL mechanism was also discussed. Under the optimum conditions, the CL intensity was linear with the concentration of dopamine from 1.2 ×10^-5to 7.8 ×10^-8 mol/L with a detection limit（ S/N = 3） of 1.5×10^-9 mol/L. The RSD（ n = 11 ） for the detection of 2.0% method was sucessfully applied to analyze dopamine in pharmaceutical and human urine.