姜黄素对心肌梗死大鼠心肌ROS、MDA及P-AKT/AKT的影响

Effect of curcumin on cardiac remodeling in rats with myocardial infarction

目的研究姜黄素对心肌梗死大鼠心肌ROS、MDA与P-AKT/AKT的表达情况。方法选60只成年雄性SD大鼠,随机分为对照组(control,n=15)、手术组(MI,n=15),溶剂组(solvent,n=15)和药物组(curcumin,n=15)。对照组在左冠状动脉前降支处穿线,打一假结。手术组、溶剂组和药物组都结扎左冠状动脉前降支,阻断其血流通过。24 h以后将三组进行不同干预,手术组不进行任何干预,溶剂组腹腔注射溶剂(乙醇和聚乙二醇的混合物),药物组腹腔注射姜黄素100 mg/kg。28 d后取出大鼠心脏,检测大鼠心肌的ROS、MDA表达含量。采用Western blot方法检测大鼠心肌组织中P-AKT/AKT的表达情况。结果心电图结果显示,手术组手术后ST变移率与T波幅度明显高于手术前(P<0.01),对照组手术前后心电图没有明显区别(P>0.05)。手术组与溶剂组大鼠心肌中ROS、MDA明显高于药物组(P<0.01),手术组与溶剂组没有明显差别(P>0.05),手术组与溶剂组大鼠心肌中ROS、MDA明显高于对照组(P<0.01)。Western blot检测P-AKT/AKT表达结果显示,手术组和溶剂组心肌梗死大鼠心肌内P-AKT/AKT表达情况明显低于药物组(P<0.05);手术组与溶剂组没有明显差别(P>0.05),手术组与溶剂组与对照组比P-AKT/AKT表达情况明显降低(P<0.05)。结论姜黄素能明显降低大鼠心肌梗死心肌内ROS、MDA的表达量;增高大鼠心肌梗死心肌内P-AKT/AKT表达。

Objective In this study we explore the effects of curcumin on ROS, MDA and P-AKT/AKT ratio in rats of myocardial infarction. Methods 60 healthy adult male SD rats were randomly divided into control group（n=15）, MI group（n=15）, solvent group（n=15） and curcumin group（n=15）, the LAD was ligated to block the blood flow in MI group, solvent group and curcumin group,whereas the blood flow in the control group was uninterrupted. After 24 hours, no further intervention was done to the control group. The solvent group was treated with only solvent without drug and curcumin group with curcumin at 100 mg/kg once daily intraperitoneally for 28 days. Test the expression of ROS,MDA and P-AKT/AKT in the myocardium after 28 days. Results There was a significant decrease（P〈0.01） in ROS and MDA in curcumin group compared to control and solvent group, and P-AKT/AKT increased（P〈0.05）. Conclusion Our experiment revealed that curcumin can effectively reduce the oxidative stress and increase P-AKT/AKT after myocardial infarction in rats.