179例血液病患者EBV和HCMV核酸检测阳性率与患者年龄的关系

Relationship between positive rate of EBV and HCMV testing with patients′age in 179 cases of leukemia

目的了解再生障碍性贫血、急性淋巴细胞白血病及髓系白血病患者EB病毒(EBV)和人巨细胞病毒(HCMV)核酸检测阳性率与其年龄的关系。方法收集中国人民解放军海军总医院2012年1月至2013年12月骨髓穿刺后临床确诊为再生障碍性贫血、急性淋巴细胞白血病及髓系白血病患者为研究对象,采用达安基因核酸检测试剂盒对EBV和HCMV核酸进行检测,分析EBV和HCMV感染阳性率与患者年龄的关系。结果 EBV和HCMV在3种血液病中核酸检测阳性率不同,HCMV核酸检测阳性检出率(15.8%)低于EBV(43.7%)。再生障碍性贫血和髓系白血病患者中,不同年龄患者EBV核酸检测阳性率比较差异有统计学意义(P<0.01)。结论对于儿童再生障碍性贫血病和髓系白血病患者应更加重视EBV和HCMV核酸的监测。

Objective To understand the relationship between the positive rate of Epstain-Barr virus（EBV）and human cytomegalovirus（HCMV）nucleic acid detection with the age in the patients with aplastic anemia,acute lymphoblastic leukemia and myeloid leukemia.Methods The patients clinically diagnosed as aplastic anemia,acute lymphoblastic leukemia,and myeloid leukemia after bone marrow puncture in the Navy General Hospital from January 2012 to December 2013 were collected as the research subjects.the nucleic acid test kit from DAAN Gene Company was adopted for conducting the EBV and HCMV nucleic acid testing and analyzing the relationship between the positive rate of the EBV and HCMV infection with the patient′s age.Results There were different testing positive rate among 3kinds of hematonosis.As a whole,the positive rate of HCMV was lower than that of EBV（15.8% vs.43.7%）.The EBV nucleic acid detection positive rate had statistically significant difference among different ages of patients with aplastic anemia and myeloid leukemia.（P〈0.01）.Conclusion For children patients with aplastic anemia disease and myeloid leukemia,more attention should be paid to monitoring of EBV and HCMV nucleic acid.