网膜囊冲洗液检测在胃癌诊治中的应用价值

Clinical application of omental bursa wash detection in gastric carcinoma

目的:初步评估胃癌患者术中网膜囊冲洗液检测的应用价值.方法:分析78例胃癌患者的临床资料,分别收集术中网膜囊冲洗液,采用流式细胞术(flow cytometry,FCM)检测网膜囊冲洗液中游离细胞的癌胚抗原(carcino-embryonic antigen,CEA)表达情况,同时行腹腔冲洗液细胞学(peritoneal lavage cytology,PLC)检查.结果:网膜囊冲洗液FCM和PLC的检测阳性率分别为37.1%(29/78)、11.5%(9/78),两者差异有统计学意义(P=0.001).胃癌网膜囊冲洗液阳性与肿瘤大小、肿瘤部位以及浸润深度密切相关(P<0.05),而与年龄、性别、分化程度、脉管癌栓、侵犯面积无关(P>0.05).结论:FCM可作为网膜囊冲洗液的快速检测手段,网膜囊冲洗液检测可评估肿瘤的进展情况,对预测腹膜微转移有一定的价值.

AIM：To evaluate the application value of omental bursa wash detection in gastric carcinoma.METHODS：Omental bursa washes from 78 patients with gastric carcinoma were collected.The expression of carcino-embryonic antigen（CEA） in omental bursa washes was detected by flow cytometry（FCM）.Peritoneal lavage cytology（PLC） examinations were also carried out.RESULTS：The FCM and PLC positive rates of omental bursa washes were 37.1%（29/78）and 11.5%（9/78）,respectively,which had a significant difference（P = 0.001）.The positive rate of omental bursa washes correlated well with the width of tumor,location of lesion,and depth of invasion（P〈0.05）.The age,gender,differentiation,vessel carcinoma embolus,and area of invasion were not relevant to omental buxsa wash detection.CONCLUSION：FCM can be well used to detect omental bursa washes.The best use of omental bursa wash detection is to assess tumor progression and to predict the emergence of gastric carcinoma micrometastases.