低剂量辐射对CIK细胞杀伤乳腺癌细胞作用的影响

Effects of low dose radiation on cytokine-induced killer cells on breast cancer cell lines

目的 研究低剂量辐射对CIK细胞杀伤乳腺癌细胞MCF-7和SK-BR-3作用的影响,探讨其可能的作用机制.方法 取健康人外周血浓缩白细胞,常规分离出单个核细胞,加入不同的细胞因子培养7d,诱导DC与CIK细胞,并将其按1∶5的比例共同培养7d获得DC-CIK细胞.DC-CIK及CIK细胞分别给予40、80、120 mGy X射线照射,流式细胞仪检测细胞表型变化,运用CCK-8的方法检测其对乳腺癌细胞的杀伤作用.结果 DC-CIK组对乳腺癌细胞SK-BR-3较CIK组在不同效靶比均有更强的杀伤活性（t=3.63、5.62、8.21、5.49,P＜0.05）.与CIK 0 mGy组相比,CIK细胞联合40、80、120 mGy低剂量辐射对乳腺癌细胞MCF-7和SK-BR-3的杀伤活性明显增高,而DC-CIK细胞联合低剂量辐射组与DC-CIK 0 mGy组相比,差异无统计学意义.结论 低剂量辐射对CIK细胞杀伤乳腺癌细胞有协同作用.

Objective To observe the effects of low dose radiation on cytokine-induced killer cells on breast cancer cell lines and explore the possible mechanism.Methods Peripheral blood mononuelear cells （PBMC） were separated from healthy human peripheral white blood cells.DCs and CIK cells were prepared from human PBMCs with different cytokines.CIK cells were cocultured with autologous DCs for 7 days at a ratio of 1 ∶ 5 to get immunologic effector DC-CIK cells.DC-CIK and CIK cells were irradiated by X-rays at different doses of 40,80,120 mGy,then their phenotypes were analyzed by flow cytometry and their cytotoxic effects on breast cancer cell lines were detected by CCK-8 assay.Results Compared with CIK group,DC-CIK group enhanced the cytotoxicity in breast cancer cells at different ratios （t =3.63,5.62,8.21,5.49,P 〈0.05）.Compared with CIK 0 mGy group,low dose irradiated CIK cells induced a higher cytotoxicity to MCF-7 and SK-BR-3 breast cancer cells.But there was no significant difference between DC-CIK 0 mGy group and low dose irradiated DC-CIK cells.Conclusions Low dose radiation has synergistic effect with CIK cells on killing breast cancer.