BAMBI在小鼠不同类型脂肪组织发育及脂肪细胞分化过程中的差异表达规律

Differential expression of bone morphogenetic protein and activin membrane-bound inhibitor in mouse adipose tissues and primary preadipocytes

目的研究BAMBI基因在小鼠不同脂肪组织发育过程中的表达规律。方法利用RT-q PCR及Western blot技术检测BAMBI基因在小鼠不同类型脂肪组织、不同发育阶段(胚胎第18天、出生第0天、第21天、第8周和第6个月)及原代前体脂肪细胞分化过程中(分化第0、3、7、11天)的差异表达情况。结果随着脂肪组织的发育,BAMBI m RNA及蛋白的表达量逐渐降低;在发育的同一时间段,BAMBI m RNA在内脏白色脂肪组织较皮下白色脂肪组织表达量高,在棕色脂肪组织中表达量最低;在小鼠前体脂肪细胞诱导分化过程中,BAMBI m RNA表达量呈时间依赖性降低。结论提示BAMBI的差异表达规律与脂肪组织类型、发育阶段以及脂肪细胞分化程度密切相关,其在脂肪组织发育过程中起着重要的作用。

Objective To investigate the expression profiles of bone morphogenetic protein and activin membrane- bound inhibitor（BAMBI） during the development of mouse adipose tissue. Methods The total RNA was extracted for real-time PCR for amplification of BAMBI m RNA from the suprascapular brown adipose tissue（BAT） and subcutaneous（inguinal） and visceral（gonadal） white adipose tissue（s WAT and v WAT, respectively） of mice at various embryonic and postnatal stages, as well as from isolated primary preadipocytes during differentiation. Results In BAT, BAMBI m RNA levels exhibited a transient increase, peaking at day 0（D0） and declined thereafter. s WAT and v WAT could be isolated from mice from postnatal D21 onwards, in which BAMBI m RNA levels were the highest and decreased at 8 weeks and 6 months. BAMBI m RNA levels were also significantly reduced in primary preadipocytes isolated from v WAT after induced differentiation. BAMBI m RNA expression level was higher in v WAT than in s WAT and BAT at the same developmental stages. Conclusion BAMBI is differentially expressed in different adipose tissues and developmental stages, which supports the hypothesis that BAMBI plays a pivotal role in the development of adipose tissues.