AFP-L3、凝血四项联合检测在原发性肝癌的临床应用

The clinical diagnostic value of combined detection of AFP-L3 and four items of blood coagulation in primary hepatocellular carcinoma

目的通过检测血清中甲胎蛋白异质(AFP-L3)、血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)及纤维蛋白原(FIB)含量,探讨其在原发性肝癌患者中的表达差异及关系。方法使用ACL TOP全自动血凝仪检测PT、APTT、TT及FIB含量。同时使用ELISA法检测血清AFP-L3的含量。结果原发性肝癌组与健康对照组比较,PT、APTT和AFP-L3检测结果有统计学意义(P<0.01),TT和FIB检测结果比较差异有统计学意义(P<0.05),其中FIB的阳性率随AFP-L3含量的增高呈上升趋势,其他几项变化不明显。结论凝血四项结果的异常和高阳性率及AFP-L3水平显著增高对原发性肝癌诊疗具有重要的临床指导作用。

Objective To discuss the differences and relationship of blood serum AFP-L3, prothromhin time （PT）, partial thromhoplastin time（APTT）, thrombin time（TT） and fibrinogen（Fib） in Primary Hepatic Cancer. Methods PT, APTT, TT and FIB were detected by ACL TOP automatic blood coagulation, and AFP-L3 was detected by ELISA. Results The values of PT, APTT and AFP-L3 in Primary Hepatic Cancer group were significantly higher than those in normal control group （P〈0.01）; the results of TT and FIB were significantly different between two groups（P〈0.05）.The positive rate of FIB showed a rising trend when the AFP-L3 content was increased,but several others did not change obviously. Conclusion The abnormal results and high positive rate of blood coagulation, and the significantly higher level of AFP- L3 have an important guiding role for diagnosis and treatment of Primary Hepatocellular Cancer.