外源NO对水分胁迫下平邑甜茶幼苗氧化损伤的缓解效应

Alleviated Effect of Exogenous Nitric Oxide on Oxidative Damage in Malus hupehensis(Pamp.)Rehd.Seedlings under Water Stress

以苹果属植物平邑甜茶水培幼苗为试验材料,采用20%PEG-6000模拟水分胁迫,进行了外源NO对水分胁迫下平邑甜茶幼苗氧化损伤的缓解效应研究。结果表明：100~500μmol·L-1外源硝普钠（SNP,NO供体）处理,能在一定程度上提高渗透胁迫下平邑甜茶叶片内SOD、POD、CAT、APX活性和ASA含量,不同程度减轻20%PEG-6000胁迫对平邑甜茶幼苗活性氧的累积和氧化损伤,其中以300μmol·L-1 SNP处理缓解效应最佳,而相对高浓度的700μmol·L-1 SNP处理则不能起到缓解作用,反而有一定的毒害作用。

Alleviatedeffects of exogenous nitric oxide on oxidative damage in Malus hupehensis（Pamp.）Rehd.seedlings were studied under water stress.The results showed that 100-500μmol·L-1 SNP（sodium nitrop russide,NO-donor）can alleviated the different degree oxidative damage under water stress,with the activities of superoxide ismutase（SOD）,peroxidase（POD）,catalase（CAT）,ascorbate peroxidase（APX）and the content of ascorbic acid（ASA）were increased in seedling＇s leaves.The study showed that 300μmol·L-1 SNP have the significantly effects and relatively high concentration（700 mmol· L-1 SNP）can not play a relief role,meantime there are certain toxic effectson M.hupehensis seedings.