摘要
The biological activity of the multifunctional cytokine interleukin-1 （IL-1） is mediated by its receptors. The aim of this study was to determine if an association exists between single nucleotide polymorphisms （SNPs） in the IL-1 type I and 2 receptor genes （ILIR1 and ILIR2） and the expression level of membrane-bound ILIRs on subpopulations of mononuclear cells or serum levels of soluble IL-1 receptors. It was observed that healthy individuals with the genotype TT in SNP rs2234650.C〉T had a lower percentage of intact CD14＋ monocytes expressing ILIR1 on their surface. The SNP rs4141134-T〉C in ILIR2 has also been associated with the percentage of intact CD3＋ T cells expressing ILIR2. Furthermore, individuals carrying the CC allele of SNP rs4141134.T〉C and the TT allele of SNP rs2071008-T〉G in ILIR2 had a lower density of ILIR2s on the surface of CD14＋ monocytes in lipopolysaccharide （LPS）-stimulated PBMC cultures. In summary, this study demonstrated that IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors （ILIR） on immunocompetent cells.
The biological activity of the multifunctional cytokine interleukin-1 （IL-1） is mediated by its receptors. The aim of this study was to determine if an association exists between single nucleotide polymorphisms （SNPs） in the IL-1 type I and 2 receptor genes （ILIR1 and ILIR2） and the expression level of membrane-bound ILIRs on subpopulations of mononuclear cells or serum levels of soluble IL-1 receptors. It was observed that healthy individuals with the genotype TT in SNP rs2234650.C〉T had a lower percentage of intact CD14＋ monocytes expressing ILIR1 on their surface. The SNP rs4141134-T〉C in ILIR2 has also been associated with the percentage of intact CD3＋ T cells expressing ILIR2. Furthermore, individuals carrying the CC allele of SNP rs4141134.T〉C and the TT allele of SNP rs2071008-T〉G in ILIR2 had a lower density of ILIR2s on the surface of CD14＋ monocytes in lipopolysaccharide （LPS）-stimulated PBMC cultures. In summary, this study demonstrated that IL-1 receptor gene polymorphisms could be one of the factors influencing the expression of membrane-bound IL-1 receptors （ILIR） on immunocompetent cells.
