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Cortical neurogenesis in adult rats after ischemic brain injury: most new neurons fail to mature 被引量:2

Cortical neurogenesis in adult rats after ischemic brain injury: most new neurons fail to mature
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摘要 The present study examines the hypothesis that endogenous neural progenitor cells isolated from the neocortex of ischemic brain can differentiate into neurons or glial cells and contribute to neural regeneration. We performed middle cerebral artery occlusion to establish a model of cerebral ischemia/reperfusion injury in adult rats. Immunohistochemical staining of the cortex 1, 3, 7, 14 or 28 days after injury revealed that neural progenitor cells double-positive for nestin and sox-2 appeared in the injured cortex 1 and 3 days post-injury, and were also positive for glial ifbrillary acidic protein. New neurons were labeled using bromodeoxyuridine and different stages of maturity were identiifed using doublecortin, microtubule-associated protein 2 and neuronal nuclei antigen immunohistochemistry. Immature new neurons coexpressing doublecortin and bromodeoxyuridine were observed in the cortex at 3 and 7 days post-injury, and semi-mature and mature new neurons double-positive for microtubule-associated protein 2 and bromode-oxyuridine were found at 14 days post-injury. A few mature new neurons coexpressing neuronal nuclei antigen and bromodeoxyuridine were observed in the injured cortex 28 days post-injury. Glial ifbrillary acidic protein/bromodeoxyuridine double-positive astrocytes were also found in the injured cortex. Our ifndings suggest that neural progenitor cells are present in the damaged cortex of adult rats with cerebral ischemic brain injury, and that they differentiate into astrocytes and immature neurons, but most neurons fail to reach the mature stage. The present study examines the hypothesis that endogenous neural progenitor cells isolated from the neocortex of ischemic brain can differentiate into neurons or glial cells and contribute to neural regeneration. We performed middle cerebral artery occlusion to establish a model of cerebral ischemia/reperfusion injury in adult rats. Immunohistochemical staining of the cortex 1, 3, 7, 14 or 28 days after injury revealed that neural progenitor cells double-positive for nestin and sox-2 appeared in the injured cortex 1 and 3 days post-injury, and were also positive for glial ifbrillary acidic protein. New neurons were labeled using bromodeoxyuridine and different stages of maturity were identiifed using doublecortin, microtubule-associated protein 2 and neuronal nuclei antigen immunohistochemistry. Immature new neurons coexpressing doublecortin and bromodeoxyuridine were observed in the cortex at 3 and 7 days post-injury, and semi-mature and mature new neurons double-positive for microtubule-associated protein 2 and bromode-oxyuridine were found at 14 days post-injury. A few mature new neurons coexpressing neuronal nuclei antigen and bromodeoxyuridine were observed in the injured cortex 28 days post-injury. Glial ifbrillary acidic protein/bromodeoxyuridine double-positive astrocytes were also found in the injured cortex. Our ifndings suggest that neural progenitor cells are present in the damaged cortex of adult rats with cerebral ischemic brain injury, and that they differentiate into astrocytes and immature neurons, but most neurons fail to reach the mature stage.
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第2期277-285,共9页 中国神经再生研究(英文版)
关键词 nerve regeneration middle cerebral artery occlusion brain injury NEURONS ASTROCYTES OLIGODENDROCYTES neural progenitor cells proliferation differentiation NEUROGENESIS neural regeneration nerve regeneration middle cerebral artery occlusion brain injury neurons astrocytes oligodendrocytes neural progenitor cells proliferation differentiation neurogenesis neural regeneration
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  • 1Abrahams JM, Gokhan S, Flamm ES, Mehler MF (2004) De novo neu- rogenesis and acute stroke: are exogenous stem cells really necessary? Neurosurgery 54:150-156.
  • 2Arvidsson A, Collin T, Kirik D, Kokaia Z, Lindvall O (2002) Neuronal replacement from endogenous precursors in the adult brain after stroke. Nat Med 8:963-970.
  • 3Burns TC, Verfaillie CM, Low WC (2009) Stem cells for ischemic brain injury: a critical review, l Comp Neurol 515:125-144.
  • 4Chen J, Guo Y, Cheng W, Chen R, Liu T, Chen Z, Tan S (2013) High glucose induces apoptosis and suppresses proliferation of adult rat neural stem cells following in vitro ischemia. BMC Neurosci 14:24.
  • 5Chen J, Venkat P, Zacharek A, Chopp M (2014) Neurorestorative thera- py for stroke. Front Hum Neurosci 8:382.
  • 6Dirnagl U (2006) Bench to bedside: the quest for quality in experimen- tal stroke research. J Cereb Blood Flow Metab 26:1465-1478.
  • 7Dong J, Liu B, Song L, Lu L, Xu H, Gu Y (2012) Neural stem cells in the ischemic and injured brain: endogenous and transplanted. Cell Tis- sue Bank 13:623-629.
  • 8El Amki M, Lerouet D, Coqueran B, Curis E, Orset C, Vivien D, Plotldne M, Marchand-Leroux C, Margaill I (2012) Experimental modeling of recombinant tissue plasminogen activator effects after ischemic stroke. Exp Neurol 238:138-144.
  • 9Gilman S (2006) Pharmacologic management of ischemic stroke: rele- vance to stem cell therapy. Exp Neurol 199:28-36.
  • 10Goldstein LB (2007) Acute ischemic stroke treatment in 2007. Circula- tion 116:1504-1514.

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