LIG4及HSPB1基因单核苷酸多态性与肺癌放射性肺损伤的相关性研究

Relationship between genetic single-nucleotide polymorphisms of LIG4 and HSPB1 and the radiation-induced lung injury in lung cancer patients

对辽宁地区170例临床确诊不可手术的肺癌接受放疗者放疗中及放疗后评价放射性肺炎发生情况。170例患者中,非小细胞肺癌(NSCLC)112例,小细胞肺癌(SCLC)58例,33例发生2级以上的放射性肺炎(19.4%)。其中2级21例,3级9例,4级1例,5级2例。男性26例(78.8%),女性7例(21.2%)。采用外周血标本酚-氯仿法提取DNA,Taqman real-time PCR方法行单核苷酸多态性的基因分型。应用SPSS 13.0软件进行数据整理分析,p<0.05时认为有统计学显著性差异。以χ2检验比较人口学特征、相关危险因素暴露及SNPs基因型在组间分布的差异;以logistic回归计算比值比(Odds ratio,OR)及95%可信区间(Confidence interval,CI)表示相对危险度。结果显示,全组一般状况和接受治疗情况均与放射性肺炎的发生无显著关联(p>0.05)。LIG4rs1805388与HSPB1rs2868371基因型分布频率在未发生放射性肺炎组和发生放射性肺炎组间分布无明显差异(p>0.05),表明LIG4rs1805388与HSPB1rs2868371位点基因单核苷酸多态性与肺癌放射性肺损伤无关。

A total of 170 lung cancer patients, who diagnosed with inoperable lung cancer and received radiotherapy, were included in the present study. DNA were extracted by phenol-chlorofrom method from blood samples. Genetic single-nucleotide polymorphisms were divided by Taqman real-time PCR. All statistical analyses were performed with statistical product and service solutions v13.0. p〈0.05 was confirmed statistically significant. We used χ^2 analysis to compare the differences in demographic characteristics, exposure to risk factors and SNPs genotypes between the two cases. Crude odds ratios（ORs） with 95% confidence intervals（95%CI） were calculated by Logistic regression analysis. Among the 170 patients, 112 suffered from non small cell lung cancer, 58 small cell lung cancer, 33 radiation-induced lung injury（≥2 grade）. 2 grade were 21 cases, 3 grade were 9 cases, 4 grade were 1 case, 5 grade were 2 cases. Male were 26 cases（78.8%）, female were 7 cases（21.2%）. There was no significant association among general characteristic, treatment and the radiation-induced lung injury. There was no obvious relationship between genetic single-nucleotide polymorphisms of LIG4 and HSPB1 and the radiation-induced lung injury in lung cancer patients.