白花九里明提取液对小鼠血压、心率和血清一氧化氮、内皮素-1浓度的影响

The effects of Blumea megacephala extract on the blood pressure,heart rate and serum NO and ET-1 concentrations of mice

目的观察白花九里明提取液对小鼠血压、心率以及血清一氧化氮(NO)、内皮素-1(ET-1)浓度的影响,初步分析白花九里明对心血管系统影响及其机制。方法取50只小鼠,随机均分为5组,正常对照组给予0.85%Na Cl注射液,美托洛尔组给予0.3 mg/m L美托洛尔溶液,替米沙坦组给予0.5 mg/m L替米沙坦溶液,白花九里明低浓度组和高浓度组分别给予150 mg/m L和300 mg/m L白花九里明提取液,灌胃每天1次,共14 d。末次灌胃20 min测量小鼠血压和心率后,摘除眼球取血,检测血清NO、ET-1浓度。结果白花九里明低浓度组的收缩压、舒张压、心率和血清NO、ET-1浓度与正常对照组比较差异无统计学意义(P>0.05)。白花九里明高浓度组的收缩压和舒张压均明显低于正常对照组和白花九里明低浓度组(P<0.05,P<0.01),但与替米沙坦组差异无统计学意义(P>0.05);白花九里明低浓度组、替米沙坦组的心率与正常对照组差异无统计学意义(P>0.05),美托洛尔组的心率明显低于正常对照组(P<0.01),白花九里明高浓度组的心率明显低于替米沙坦组(P<0.01)而与正常对照组和美托洛尔组差异无统计学意义(P>0.05)。血清NO浓度:仅有白花九里明高浓度组明显高于正常对照组(P<0.05),其余组间比较差异无统计学意义(P>0.05);血清ET-1浓度:仅有白花九里明高浓度组和替米沙坦组明显低于正常对照组(P<0.05,P<0.01),其余组间比较差异无统计学意义(P>0.05)。结论白花九里明达到一定用量能降低动脉血压,但对心率没有明显影响,其降压作用可通过减少心搏出量和降低外周阻力来实现。

Objective To investigate the effects of blumea megacephala extract on the blood pressure , heart rate and serum NO and ET-1 concentrations of mice , thus to preliminarily analyze the mechanism of Blumea megacephala ex-tract on the cardiovascular system .Methods Fifty mice were randomly divided into normal control group , metoprolol group, telmisartan group , Blumea megacephala low-concentration group and high -concentration group , in which differ-ent lavages were given to for 14 days.Blood pressure, heart rate and serum NO and ET -1 concentrations were were de-termined aftere that .Results There was no significant difference in the systolic blood pressure , diastolic blood pressure or serum NO and ET -1 concentrations between the Blumea megacephala low -concentration group and normal control group.Significantly lower systolic blood pressure and diastolic blood pressure were observed in Blumea megacephala high-concentration group than those in normal control group and Blumea megacephala low-concentration group （ P〈0.05, P〈0.01） , and with no significant difference comparing with telmisartan group .There was no significant difference in heart rate between normal control group and Blumea megacephala low-concentration group or telmisartan group , however , the heart rate in metoprolol group was significantly lower normal control group （ P〈0.01 ） .The heart rate in Blumea megacephala high-concentration group was significantly lower than that in telmisartan group （ P〈0.01） , with no signifi-cant difference comraring with normal control group .Serum NO concentration in Blumea megacephala high-concentration group was significantly higher than normal control group （ P〈0.05 ） , while no significant difference revealed among the other 3 groups .Serum ET-1 concentrationsin Blumea megacephala high-concentration group and telmisartan group were significantly lower than normal control group （P〈0.05, P〈0.01）, while there was no significant difference revealed a-mong the other groups .Conclusion Blumea megacephala in a certain dosage can reduce blood pressure , without appar-ent effect on heart rate , which probably via reduction in stroke volume and decreased peripheral vascular resistance .