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胰岛素样生长因子Ⅱ受体在病毒性心肌炎小鼠心肌中的表达 被引量：1

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摘要 目的观察胰岛素样生长因子Ⅱ受体(IGF-ⅡR)在病毒性心肌炎(VMC)小鼠心肌组织中的动态表达并探讨其与心肌损伤的关系。方法随机将120只雄性4周龄BALB/c小鼠分为两组:VMC组80只,对照组40只。VMC组腹腔接种含柯萨奇B3病毒的DMEM液建立VMC模型,对照组接种等量不含病毒的DMEM液。于感染后第3、7、14、28天每组各取10只收集心脏标本,HE染色观察心肌病理改变,免疫组织化学技术测定心肌IGF-ⅡR蛋白表达水平,RT-PCR技术测定心肌IGF-ⅡR mRNA的相对表达量;分析IGF-ⅡR的动态表达水平及其与心肌病变程度的相关性。结果 VMC组小鼠感染病毒后第3天心肌组织中的IGF-ⅡR蛋白表达开始增多,第7天达到高峰。VMC组小鼠心肌组织中IGF-ⅡR的蛋白表达水平在各个时间点均高于对照组,差异有统计学意义(P<0.05),且IGF-ⅡR蛋白表达水平与心肌病理学评分呈正相关。结论 IGF-ⅡR的表达上调可能在VMC小鼠心肌损伤中起一定作用。
作者 陈元元 范晓晨
机构地区 安徽医科大学第一附属医院儿科
出处 《广东医学》 CAS 北大核心 2015年第6期832-835,共4页 Guangdong Medical Journal
基金 安徽省中医药科研课题(编号:2009ZY36)
关键词 病毒性心肌炎 小鼠 胰岛素样生长因子Ⅱ受体 免疫组织化学
分类号 R735.7 [医药卫生—肿瘤]
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参考文献13

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二级参考文献12

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广东医学
2015年 第6期

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