自污染染料中筛选的菌对颜料紫1的脱色

Decoloration of pigment violent 1 by the strain isolated from stained dye

为研究颜料紫1的脱色问题,从一盒长满菌苔的广告浓缩染料(主要显色原料颜料紫1)中筛选菌株,通过摇瓶培养、以及平板分离等获得了单一纯种菌株、并进一步对该菌株进行了18S r DNA测序分析,从而鉴定出该菌为杂色曲霉(Aspergillus versicolor)。在此基础上,系统地研究了该菌株对颜料紫1的脱色条件。综合单因素实验和响应面法优化实验的结果表明:该杂色曲霉菌对于颜料紫1脱色的最佳条件为碳源葡萄糖30g/L,颜料紫1起始含量为250 mg/L,Na NO3含量为0.3g/L,p H值5.5,这时可获得最高的颜料紫1脱色率为84.64%。最后,通过傅里叶变换红外光谱仪(FTIR)分析,揭示了该杂色曲霉菌对颜料紫1的脱色机制主要为吸附脱色。

To investigate the decoloration problem of pigment violent 1,a strain was isolated from advertising dye,whose main composition was pigment violent 1. A single and pure strain was first obtained by flask shake and dish culture methods. 18S rDNA gene sequence analysis identified that the strain was Aspergillus versicolor.Then the decoloration conditions of pigment violent 1 were studied systematically by this novel strain. The optimal condition was found by mono factor experiment and response surface experiment as the glucose 30g/L,the original concentration of pigment violent 1 250 mg/L,the concentration of NaNO3 0.3g/L,and the pH 5.5. Under the optimal decoloration condition, the decoloration rate of pigment violent 1 reached 84.64% by the strainAspergillus versicolor. The discoloration mechanism of pigment violent 1 was analyzed and characterized by FTIR,and the results indicated that the main mechanism of the decoloration was bio-adsorption.