大负荷跑台运动对大鼠心肌细胞膜Na^+-K^+-ATPase、Ca^(2+)-ATPase与肌浆网Ca^(2+)-ATPase活性的影响

Influence of Na^+- K^+- ATPase,a Large Load of Treadmill Exercise on Myocardial Cellmembrane of Rat Ca^(2+)- ATPase and Sarcoplasmic Reticulum Ca^(2+)- ATPase Activity

目的:探讨大负荷跑台运动对大鼠心肌细胞膜钠钾泵(Na+-K+-ATPase)、钙泵(Ca2+-ATPase)与肌浆网钙泵(Ca2+-ATPase)活性的影响。方法:选择30只成年雄性SD大鼠分为空白对照组和运动后24 h组以及运动后即刻组。结果:运动后即刻组的Na+-K+-ATPase、Ca2+-ATPase活性均显著低于其他两组,(P<0.05);运动后24 h组的Ca2+-ATPase显著低于其他两组,(P<0.05);电镜观察可见运动后24 h组的肌细胞及肌浆网出现肿胀,肌原纤维结构出现明显的损伤;运动后即刻组的线粒体结构出现明显的损伤。结论:大负荷跑台运动会导致大鼠心肌细胞膜钠钾泵(Na+-K+-ATPase)、钙泵(Ca2+-ATPase)与肌浆网钙泵(Ca2+-ATPase)活性的降低。

Objective： To explore the influence of large treadmill exercise on sodium and potassiumin rat myocardial cell membrane pump（ Na＋- K＋- ATPase） and calcium pump（ Ca2 ＋- ATPase） and s-arcoplasmic reticulum calcium pump（ Ca2 ＋- ATPase） activity. Method： 30 grown male SD rats wer-e slected and were divided into norn＇lfll contrapositivemn group,24 hour after exercise group and instantly after exercise group. Results： Na＋- K＋- ATPase,Ca2 ＋- ATPase activity of the immediately after exercise group was significant lower than t-hat of the other two groups,（ all P〈0. 05）. Ca2 ＋- ATPase activity of the24 hour after exercise group was significant lower than that of the other t-wo groups,（ P〈0. 05）. Muscle cells and sarcoplasmic reticulum of the 24 group h after exercise were swelling observaed by the electron microscopy and obvious injury were appeared of the myo-fibrillar structure. Obvious injury were appeared of the structure of mitochondria of the immediately after exercise group. Conclusion： Large treadmi-ll exercise can lead to the reduce of sodium a-nd potassium in rat myocardial cell membrane p-ump（ Na＋- K＋- ATPase） and calcium pump（ Ca2 ＋-ATPase） and sarcoplasmic reticulum calcium pump（ Ca2 ＋- ATPase） activity.