苦豆子总碱对大鼠离体子宫平滑肌收缩的影响

Contraction Effects of Total Alkali Sophora Alopecuroides on Rat's Uterine Smooth Muscle Strips in Vitro

目的:探讨苦豆子总碱(total alkali sophora alopecuroids L,TASa)对大鼠离体子宫平滑肌自发收缩的影响及机制。方法:将大鼠离体子宫平滑肌置于恒温灌流肌槽中,累积加入不同浓度TASa溶液,记录肌肉收缩活动的变化,并观察其量效关系(r);分为正常组、缩宫素、TASa、TASa+异搏定、TASa+酚妥拉明、TASa+苯海拉明、TASa+消炎痛和TASa+阿托品8组以探讨其作用机制。结果:TASa能增强子宫平滑肌的收缩幅度、收缩时程和收缩面积,并有量效依赖性(幅度:r=0.99,t=21.54,P<0.01;时程:r=0.93,t=11.98,P<0.01;收缩面积:r=0.94,t=11.24,P<0.01),但对收缩频率(次/5 min)无影响(正常组:3.93±0.12,TASa:3.92±0.08,P>0.05);TASa增强子宫平滑肌收缩时程和收缩面积的效应强于缩宫素;异搏定和酚妥拉明可减弱TASa收缩平滑肌的效应,而阿托品、苯海拉明和消炎痛则无影响。结论:TASa能增强子宫平滑肌的收缩作用,其机制可能是通过L-钙通道和α受体升高胞浆内Ca2+有关。

Objective：To study the spontaneous contraction effects of total alkali sophora alopecuroidesL（TASa）on rat＇s uterine smooth muscle strips in vitro and the possible mechanisms. Method：Put the rat＇s uterine smooth muscle trips into constant temperature perfusion of the bath machine,give TASa of different dose to uterine smooth muscle,record amplitude,duration,area and contraction frequency of the uterine smooth muscle,and observed the dose-effect relationship between muscle contraction and different doses TASa. Uterine smooth muscle were divided into eight groups,including control group,Oxytocin group,TASa group,TASa＋ verapamil group,TASa＋ phentolamine group,TASa＋ benzhydramine group,TASa＋ indomethacin group,TASa＋ atropine group. The contraction mechanism of TASa were investigated. Results：TASa can increase uterine smooth muscle contraction amplitude,duration and area,and has an obvious concentration-dependent contraction（contraction amplitude r=0.99,t=21.54,P〈0.01;contraction duration r=0.93,t=11.98,P〈0.01;contraction area r=0.94,t=11.24,P0.01,and TASa has no effect in contraction frequency）. Isoptin and atropine can inhibit the uterine smooth muscle contraction of TASa,benzhydramine,indomethacin,and phetolamine have no contraction effect（control group 3.93±0.12;TASa group 3.92±0.08,P〈0.05）;TASa can increase contraction duration and area of the uterine smooth muscle better than Oxytocin;verapamil and phentolamine can inhibit the uterine smooth muscle contraction of TASa,benzhydramine,indomethacin and atropine have no contraction effect. Conclusion：TASa can increase the uterine smooth muscle＇s contraction in vitro,and its mechanism might be increased calcium flow to the interior of the cell through activating L-calcium channel and α- receptor.