HPLC测定白鲜皮配方颗粒中梣酮含量

Determination of Fraxinellone in Dittany Bark Dispensing Granules by HPLC

目的：考察HPLC测定白鲜皮配方颗粒中梣酮含量的分析方法。方法：DIONEX Acclaim？120C18（4.6mm×250mm,5μm）色谱柱,以甲醇-水（55∶45）为流动相;检测波长为236nm。结果：梣酮的线性范围为0.06056~2.4224μg,含量为42mg/g,回收率为98.5%,RSD为0.86%。结论：该法简便准确,适用于白鲜皮配方颗粒的质量控制。

Objective：To determine the content of Fraxinellone in Dittany bark dispensing granules by HPLC.Methods：DIONEX Acclaim？120C18（4.6mm×250mm,5μm）column was used,Mobile phase consisted of methanol-Water（55∶45）;UV detector wavelength was set at 236 nm.Results：The linearity was from 0.06056 to 2.4224μg for fraxinellone,The content is more than 42mg/g,the average recovery was 99.8%,RSD 1.39%.Conclusion：The method is handy and accurate,and can be used for the quality control of Dittany bark dispensing granules