油桐乙酰辅酶A羧化酶BC亚基全长cDNA克隆及序列分析

Cloning and sequence analysis of BC gene from Vernicia fordii

以葡萄桐的近成熟种子为材料,根据油桐转录组测序结果设计引物,采用RT-PCR技术克隆了油桐乙酰辅酶A羧化酶BC亚基基因的全长c DN A序列。该基因c DNA序列全长1 605 bp,编码534个氨基酸。推导出的BC亚基氨基酸序列蛋白的等电点p I为7.98,相对分子量58 262.0 Da,稳定系数为38.13,生物信息学分析表明,此蛋白含有2个比较明显的跨膜区,是一个不稳定的非分泌蛋白。模体搜索结果表明在BC上具有ATP结合位点。三级结构中有16个α螺旋,3个部分形成一个内凹的结构。

With nearly ripe Puputung tree seeds as the tested materials, the pri mers were designed according to the results of the transcriptome sequencing analysis of Vernicia fordii, and a full-length c DNA of BC gene was cloned by RT-PCR technique from nearly ripe seed in Vernicia fordii. The c DNA sequence length of BC gene is 1605 bp, encoding 534 amino acids. BC protein＇s isoelectric point（p I） is 7.98 and the molecular mass of the protein is 58262.0 Da, it＇s stability factor is 38.13. According to bioinformatics analysis, the protein bears two trans-membran e domains, and is a unstable protein. There is a ATP-grasp fold profile in BC. There are 16 alpha helixes in the tertiary structure and there is a hollow concave structure in the central of this protein.