恶性肿瘤患者血液HBV-M与HBV DNA的检测结果分析

Analysis of HBV-M and HBV DNA in serum of malignant tumor patient

目的探讨恶性肿瘤患者乙型肝炎病毒(HBV)血清标志物(HBV-M)和HBV DNA检测结果及临床意义。方法采用化学发光免疫分析和荧光定量PCR方法,对所有病例及对照组进行HBV-M和HBV DNA检测。结果 405例各种恶性肿瘤患者HBV-M阳性共185例,阳性率为45.7%,HBV DNA检出率大三阳组为100%(19/19),小三阳组为60.0%(15/25),二者比较具有显著性的统计学意义(P<0.05)。肝癌患者的阳性率最高,达89.2%;其次为胃、食管、直肠癌,其阳性率分别为48.9%、45.5%、45.2%;白血病为35.8%;肺、鼻咽癌分别为32.4%、25.0%;(恶性)淋巴瘤、甲状腺癌、宫颈癌的阳性率分别为32.0%、28.8%、23.8%。与对照组比较,差异有统计学意义(P<0.01),恶性消化道肿瘤患者HBV感染的阳性率与其他系统恶性肿瘤患者相比明显偏高(P<0.05)。结论恶性肿瘤患者的HBV感染阳性率高,其中消化道恶性肿瘤的HBV感染阳性率最高。

Objective To investigate hepatitis B virus （HBV） markers in the malignant tumor patients serum （HBV-M） and HBV DNA detection results and their clinical significance. Methods Chemiluminescence immunoassay and fluorescence quantitative PCR method were used in detection of the patients and control group in HBV-M and HBV DNA. Results In 405 patients with HBV-M positive,were 185 cases of malignant tumor, the positive rate was 45.7%;HBV DNA positive rate of HBeAg group was 100%（19/19）;Small ＂Sanyang＂ group was 60%（15/25）,Statistical significance among the two groups was significant （P〈0.05）. The positive rate of liver cancer patients was with the highest,up to 89.2%;Secondary,esophageal,gastric cancer,i.e positive rates were 48.9%,45.5%,45.2%;Leukemia was 35.8%; lung,nasopharyngeal carcinoma were 32.4%,25%;（malignant）,the positive rate of lymphoma of thyroid cancer,cervical cancer were 32%,28.8%,23.8% respectively. Compared with the control group,the difference was statistically significant （P〈0.01）,malignant tumor of digestive tract in patients infected with HBV positive rate is significantly higher than patients with other malignant tumors （P〈0.05）. Conclusion HBV in patients with malignant tumor infection positive rate is high,the malignant tumor of digestive tract HBV infection positive rate is the highest.