HPLC测定中药巴豆中巴豆苷和木兰花碱的含量

Determination of Isoguanosine and Magnoflorine in Crotonis Fructus by HPLC

目的：建立HPLC测定巴豆中巴豆苷和木兰花碱含量的方法,比较不同产地样品含量差异为制定中药巴豆的质量标准提供实验依据。方法：采用Accurasil C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈（A）-0.2%磷酸0.2%三乙胺水溶液（B）为流动相,梯度洗脱（0-10 min,5%A;10-12 min,5%-15%A;12-29 min,15%A;29-30 min,15%-5%A）,流速0.8m L·min-1,柱温30℃,检测波长275 nm。结果：巴豆苷在0.078 8-0.472 8μg线性关系良好（r=0.999 9）,平均加样回收率为99.29%,RSD 2.5%;木兰花碱在0.013 7-0.082 2μg线性关系良好（r=0.999 9）,平均加样回收率为99.28%,RSD2.2%。不同产地巴豆中巴豆苷、木兰花碱的含量差异较大。巴豆苷的含量以四川最高、广西最低,分别为17.57,7.07 mg·g^-1,木兰花碱的含量以贵州最高、广西最低,分别为3.42,1.00 mg·g^-1。结论：该方法准确简便,快速可行,可用于中药巴豆的质量控制。

Objective： An HPLC assay was established to determine and compare the contents of isoguanosine and magnoflorine in Crotonis Fructus from different areas, and used for its quality control. Method： An Accurasil C18 column （4.6 mm×250 mm,5μm） was used with acetonitrile （A） -0.2% phosphoric acid- 0.2% triethylamine solution （B） as the mobile phase （0-10 min, 5%A; 10-12 min, 5%-15%A; 12-29 min, 15% A; 29-30 min, 15%-5% A）. The flow rate was 0. 8 mL ·min^-1. The column temperature was kept at 30℃. The detection wavelength was set at 275 nm. Result： Isoguanosine had good linearity in the range of 0. 078 8- 0. 472 8μg （r = 0. 999 9）. The average recovery was 99.29% with RSD of 2.5%. Magnoflorine had good linearity in the range of 0. 013 7-0. 082 2 μg （r = 0. 999 9）. The average recovery was 99.28% with RSD of 2.2%. The content of isoguanosine and magnoflorine in Crotonis Fructus from different areas varied notably. The content of isoguanosine from Siehuan was the highest with 17.57 mg ·g^-1, while the one from Guangxi was the lowest with 7.07 mg·g^-1 And the Guizhou one was the highest in the content of magnoflorine with 3.42 mg ·g^-1, while the Guangxi one was the lowest with 1.00 mg·g^-l. Conclusion： The method is convenient, rapid and accurate, which can be applied to the quality control of Crotonis Fructus.