白亮独活的HPLC指纹图谱

HPLC Fingerprint of Heracleum candicans Roots

目的:建立白亮独活的HPLC指纹图谱分析方法并测定药材中佛手柑内酯及欧前胡素含量,为该药材的质量评价提供参考。方法:收集10批白亮独活样品,采用HPLC建立指纹图谱方法,流动相水-甲醇梯度洗脱,检测波长为302 nm。利用"中药色谱指纹图谱相似度评价系统"2004A版软件进行指纹图谱分析。结果:10批白亮独活的HPLC指纹图谱相似度均>0.89,标定了11个共有峰。样品中佛手柑内酯、欧前胡素平均质量分数分别为0.117%和0.709%。结论:该方法简单、重复性好,可用于白亮独活的品种鉴别和质量评价。

Objective： To establish an effictive quality evaluation method of Heracleum candicans roots and determine contents of two active ingredients of bergapten and imperatorin in this medicinal herb. Method ： Ten batches of H. candicans roots were collected, HPLC fingerprint was performed in a gradient mode of water- methanol, detection wavelength of 302 rim. Similarity was evaluated with similarity evaluation system for chromatographic fingerprint of tradition Chinese medicine （2004A） issued by state pharmacopoeia committee. Result： HPCL fingerprint similarity among 10 batches of H. candicans roots were more than 0. 89 and 11 characteristic common peaks were demarcated. Contents of bergapten and imperatorin in samples were 0. 117% and 0. 709%. Conclusion： This method is simple and reproducible that can be used for identification and quality evaluation of H. candicans roots.