车前子提取物部位群抗痛风的作用

Anti-gout Effect of Various Fractions from Plantaginis Semen

目的:探讨车前子部位群的抗痛风活性与机制,为后续研究提供参考依据。方法:以系统溶剂法提取分离车前子。90只昆明种小鼠,随机分为9组,每组10只,分别为正常组,高尿酸血症组(模型组),别嘌呤醇组(0.01 g·kg-1),总提物组(25 g·kg-1),石油醚组(25 g·kg-1),二氯乙烷组(25 g·kg-1),乙酸乙酯组(25 g·kg-1),正丁醇组(25 g·kg-1),水部位组(25 g·kg-1)。除正常组外,其余各组采用氧嗪酸钾盐诱导小鼠急性高尿酸血症模型,ig给予相应药物,正常组和模型组给予等体积生理盐水,连续给药7 d。观察车前子总提物及5个部位对小鼠血清尿酸水平及肝脏黄嘌呤氧化酶(XOD)活性的影响。结果:系统溶剂法提取分离车前子得到5个部位,分别为石油醚、二氯甲烷、乙酸乙酯、正丁醇、水部位。与正常组比较,模型组小鼠血清尿酸的水平与XOD活性明显升高(P<0.01);与模型组比较,别嘌呤醇、车前子总提物及各不同部位均可降低小鼠血清尿酸的水平与XOD活性(P<0.05,P<0.01)。结论:车前子及不同提取部位具有一定的抗痛风作用。

Objective： To study the anti-gout effect and mechanism of Plantaginis Semen （PS） extract parts on the level of uric acid in hyperuricemia mice. Method： Five fractions including the petrol ether extract, the dichloromethane extract, the ethyl acetate extract, the butanol extract and the water-soluble extract were obtained by systematic solvent extraction. 90 Kunming mice were randomly divided into 9 groups ： the normal group （normal saline）, the hyperuricemia group （normal saline）, the allopurinol group （0.01 g ·kg^-1）, the total extract of PS （TPS, 25 g ·kg^-1） and the five fractions groups （25 g·kg^-1 ）. The hyperuricemia mice were induced by using oxygen oxazine acid potassium. The mice were administrated of the corresponding medicines for 7 days, and then the level of uric acid and the activity of xanthine oxidase were observed. Result： TPS and five fractions were obtained. Compared with the normal group, the level of uric acid and the activity of xanthine oxidase increased obviously in the hyperuricemia group （P 〈 0.01 ）. All treatment groups could reduce the level of serum uric acid obviously, and inhibit the activity of xanthine oxidase of hyperuricemia mice （P 〈0.05, P 〈0.01 ）. Conclusion： The PS and its different extracts have good anti-gout effect in hyperuricemia mice.